7ZC4
Cryo-EM structure of POLRMT mutant.
Summary for 7ZC4
| Entry DOI | 10.2210/pdb7zc4/pdb |
| EMDB information | 14619 |
| Descriptor | DNA-directed RNA polymerase, mitochondrial (1 entity in total) |
| Functional Keywords | mitochondria, dna dependent rna polymerase., transcription |
| Biological source | Homo sapiens (human) |
| Total number of polymer chains | 1 |
| Total formula weight | 134327.70 |
| Authors | Das, H.,Hallberg, B.M. (deposition date: 2022-03-25, release date: 2023-02-01, Last modification date: 2024-07-24) |
| Primary citation | Zhu, X.,Xie, X.,Das, H.,Tan, B.G.,Shi, Y.,Al-Behadili, A.,Peter, B.,Motori, E.,Valenzuela, S.,Posse, V.,Gustafsson, C.M.,Hallberg, B.M.,Falkenberg, M. Non-coding 7S RNA inhibits transcription via mitochondrial RNA polymerase dimerization. Cell, 185:2309-2323.e24, 2022 Cited by PubMed Abstract: The mitochondrial genome encodes 13 components of the oxidative phosphorylation system, and altered mitochondrial transcription drives various human pathologies. A polyadenylated, non-coding RNA molecule known as 7S RNA is transcribed from a region immediately downstream of the light strand promoter in mammalian cells, and its levels change rapidly in response to physiological conditions. Here, we report that 7S RNA has a regulatory function, as it controls levels of mitochondrial transcription both in vitro and in cultured human cells. Using cryo-EM, we show that POLRMT dimerization is induced by interactions with 7S RNA. The resulting POLRMT dimer interface sequesters domains necessary for promoter recognition and unwinding, thereby preventing transcription initiation. We propose that the non-coding 7S RNA molecule is a component of a negative feedback loop that regulates mitochondrial transcription in mammalian cells. PubMed: 35662414DOI: 10.1016/j.cell.2022.05.006 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.24 Å) |
Structure validation
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