7ZBU

CryoEM structure of SARS-CoV-2 spike monomer in complex with neutralising antibody P008_60

7ZBU の概要

• エントリーDOI: 10.2210/pdb7zbu/pdb
• EMDBエントリー: 14591
• 分子名称: Spike glycoprotein, P008_60 antibody, Heavy chain, P008_60 antibody, Light chain, ... (5 entities in total)
• 機能のキーワード: covid-19, sars-cov-2, spike, neutralizing antibody, immunity, viral protein
• 由来する生物種: Severe acute respiratory syndrome coronavirus 2; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 194171.36

構造登録者

Rosa, A.,Pye, V.E.,Cronin, N.,Cherepanov, P. (登録日: 2022-03-24, 公開日: 2022-08-17, 最終更新日: 2024-10-16)

主引用文献

Seow, J.,Khan, H.,Rosa, A.,Calvaresi, V.,Graham, C.,Pickering, S.,Pye, V.E.,Cronin, N.B.,Huettner, I.,Malim, M.H.,Politis, A.,Cherepanov, P.,Doores, K.J.
A neutralizing epitope on the SD1 domain of SARS-CoV-2 spike targeted following infection and vaccination.
Cell Rep, 40:111276-111276, 2022

PubMed Abstract: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike is the target for neutralizing antibodies elicited following both infection and vaccination. While extensive research has shown that the receptor binding domain (RBD) and, to a lesser extent, the N-terminal domain (NTD) are the predominant targets for neutralizing antibodies, identification of neutralizing epitopes beyond these regions is important for informing vaccine development and understanding antibody-mediated immune escape. Here, we identify a class of broadly neutralizing antibodies that bind an epitope on the spike subdomain 1 (SD1) and that have arisen from infection or vaccination. Using cryo-electron microscopy (cryo-EM) and hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS), we show that SD1-specific antibody P008_60 binds an epitope that is not accessible within the canonical prefusion states of the SARS-CoV-2 spike, suggesting a transient conformation of the viral glycoprotein that is vulnerable to neutralization.

PubMed: 35981534
DOI: 10.1016/j.celrep.2022.111276

実験手法

ELECTRON MICROSCOPY (4.31 Å)