7ZBF

Crystal structure of native Iripin-4 serpin from tick Ixodes ricinus

7ZBF の概要

• エントリーDOI: 10.2210/pdb7zbf/pdb
• 関連するPDBエントリー: 7zas
• 分子名称: Iripin-4 serpin, NICKEL (II) ION (3 entities in total)
• 機能のキーワード: ixodes ricinus, iripin-4, crystal, native conformation, serpin, immunosuppressant
• 由来する生物種: Ixodes ricinus (castor bean tick)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 42047.44

構造登録者

Kascakova, B.,Kuta Smatanova, I.,Chmelar, J.,Prudnikova, T. (登録日: 2022-03-23, 公開日: 2023-04-05, 最終更新日: 2024-02-07)

主引用文献

Kascakova, B.,Kotal, J.,Havlickova, P.,Vopatkova, V.,Prudnikova, T.,Grinkevich, P.,Kuty, M.,Chmelar, J.,Kuta Smatanova, I.
Conformational transition of the Ixodes ricinus salivary serpin Iripin-4.
Acta Crystallogr D Struct Biol, 79:409-419, 2023

PubMed Abstract: Iripin-4, one of the many salivary serpins from Ixodes ricinus ticks with an as-yet unexplained function, crystallized in two different structural conformations, namely the native partially relaxed state and the cleaved serpin. The native structure was solved at a resolution of 2.3 Å and the structure of the cleaved conformation was solved at 2.0 Å resolution. Furthermore, structural changes were observed when the reactive-centre loop transitioned from the native conformation to the cleaved conformation. In addition to this finding, it was confirmed that Glu341 represents a primary substrate-recognition site for the inhibitory mechanism. The presence of glutamate instead of the typical arginine in the P1 recognition site of all structurally characterized I. ricinus serpins (PDB entries 7b2t, 7pmu and 7ahp), except for the tyrosine in the P1 site of Iripin-2 (formerly IRS-2; PDB entry 3nda), would explain the absence of inhibition of the tested proteases that cleave their substrate after arginine. Further research on Iripin-4 should focus on functional analysis of this interesting serpin.

PubMed: 37092969
DOI: 10.1107/S2059798323002322

実験手法

X-RAY DIFFRACTION (2.3 Å)