7Z9D

Deinococcus radiodurans BphP PAS-GAF H260A mutant

7Z9D の概要

• エントリーDOI: 10.2210/pdb7z9d/pdb
• 関連するPDBエントリー: 5K5B 5NFX
• 分子名称: Bacteriophytochrome, ACETATE ION, (4S)-2-METHYL-2,4-PENTANEDIOL, ... (5 entities in total)
• 機能のキーワード: kinase, photosensor, transferase, phytochrome
• 由来する生物種: Deinococcus radiodurans R1
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 38043.36

構造登録者

Takala, H. (登録日: 2022-03-21, 公開日: 2022-08-10, 最終更新日: 2024-11-13)

主引用文献

Lehtivuori, H.,Rumfeldt, J.,Mustalahti, S.,Kurkinen, S.,Takala, H.
Conserved histidine and tyrosine determine spectral responses through the water network in Deinococcus radiodurans phytochrome.
Photochem Photobiol Sci, 21:1975-1989, 2022

PubMed Abstract: Phytochromes are red light-sensing photoreceptor proteins that bind a bilin chromophore. Here, we investigate the role of a conserved histidine (H260) and tyrosine (Y263) in the chromophore-binding domain (CBD) of Deinococcus radiodurans phytochrome (DrBphP). Using crystallography, we show that in the H260A variant, the missing imidazole side chain leads to increased water content in the binding pocket. On the other hand, Y263F mutation reduces the water occupancy around the chromophore. Together, these changes in water coordination alter the protonation and spectroscopic properties of the biliverdin. These results pinpoint the importance of this conserved histidine and tyrosine, and the related water network, for the function and applications of phytochromes.

PubMed: 35906527
DOI: 10.1007/s43630-022-00272-6

実験手法

X-RAY DIFFRACTION (1.88 Å)