7Z6K

CRYSTAL STRUCTURE OF WEISSELLA VIRIDESCENS FEMXVV NON-RIBOSOMAL AMINO ACID TRANSFERASE IN COMPLEX WITH A PEPTIDYL-XNA CONJUGATE

Summary for 7Z6K

• Entry DOI: 10.2210/pdb7z6k/pdb
• Related: 7Z5Y 7Z5Z 7Z6A
• Related PRD ID: PRD_002409
• Descriptor: UDP-N-acetylmuramoylpentapeptide-lysine N(6)-alanyltransferase, 2'F-ANA (5'-D(P*(A5L)P*(CFL)P*(CFL))-R(P*(A9Z))-3'), UDP-MurNAc-pentapeptide, ... (7 entities in total)
• Functional Keywords: femx, xeno-nucleic acids, peptidoglycan, transferase, peptidyl-xna conjugate, transferase-peptide-xna complex
• Biological source: Weissella viridescens; More
• Total number of polymer chains: 3
• Total formula weight: 41774.84

Authors

Li de la Sierra-Gallay, I. (deposition date: 2022-03-11, release date: 2022-10-26, Last modification date: 2024-10-23)

Primary citation

Rietmeyer, L.,Li De La Sierra-Gallay, I.,Schepers, G.,Dorchene, D.,Iannazzo, L.,Patin, D.,Touze, T.,van Tilbeurgh, H.,Herdewijn, P.,Etheve-Quelquejeu, M.,Fonvielle, M.
Amino-acyl tXNA as inhibitors or amino acid donors in peptide synthesis.
Nucleic Acids Res., 50:11415-11425, 2022

PubMed Abstract: Xenobiotic nucleic acids (XNAs) offer tremendous potential for synthetic biology, biotechnology, and molecular medicine but their ability to mimic nucleic acids still needs to be explored. Here, to study the ability of XNA oligonucleotides to mimic tRNA, we synthesized three L-Ala-tXNAs analogs. These molecules were used in a non-ribosomal peptide synthesis involving a bacterial Fem transferase. We compared the ability of this enzyme to use amino-acyl tXNAs containing 1',5'-anhydrohexitol (HNA), 2'-fluoro ribose (2'F-RNA) and 2'-fluoro arabinose. L-Ala-tXNA containing HNA or 2'F-RNA were substrates of the Fem enzyme. The synthesis of peptidyl-XNA and the resolution of their structures in complex with the enzyme show the impact of the XNA on protein binding. For the first time we describe functional tXNA in an in vitro assay. These results invite to test tXNA also as substitute for tRNA in translation.

PubMed: 36350642
DOI: 10.1093/nar/gkac1023

Experimental method

X-RAY DIFFRACTION (1.6 Å)