7Z6A

CRYSTAL STRUCTURE OF WEISSELLA VIRIDESCENS FEMXVV NON-RIBOSOMAL AMINO ACID TRANSFERASE IN COMPLEX WITH A PEPTIDYL-XNA CONJUGATE

7Z6A の概要

• エントリーDOI: 10.2210/pdb7z6a/pdb
• 関連するPDBエントリー: 7Z5Y 7Z5Z 7Z6K
• 分子名称: UDP-N-acetylmuramoylpentapeptide-lysine N(6)-alanyltransferase, UDP-MurNAc-pentapeptide, 2'F-RNA (5'-D(*(GF2)P*(GF2)P*(CFZ)P*(CFZ)P*(AF2)P*(CFZ)P*(CFZ))-R(P*(A9Z))-3'), ... (7 entities in total)
• 機能のキーワード: femx, xeno-nucleic acids, peptidoglycan, transferase, peptidyl-xna conjugate, transferase-peptide-xna complex
• 由来する生物種: Weissella viridescens; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 43083.58

構造登録者

Li de la Sierra-Gallay, I. (登録日: 2022-03-11, 公開日: 2022-10-26, 最終更新日: 2024-10-09)

主引用文献

Rietmeyer, L.,Li De La Sierra-Gallay, I.,Schepers, G.,Dorchene, D.,Iannazzo, L.,Patin, D.,Touze, T.,van Tilbeurgh, H.,Herdewijn, P.,Etheve-Quelquejeu, M.,Fonvielle, M.
Amino-acyl tXNA as inhibitors or amino acid donors in peptide synthesis.
Nucleic Acids Res., 50:11415-11425, 2022

PubMed Abstract: Xenobiotic nucleic acids (XNAs) offer tremendous potential for synthetic biology, biotechnology, and molecular medicine but their ability to mimic nucleic acids still needs to be explored. Here, to study the ability of XNA oligonucleotides to mimic tRNA, we synthesized three L-Ala-tXNAs analogs. These molecules were used in a non-ribosomal peptide synthesis involving a bacterial Fem transferase. We compared the ability of this enzyme to use amino-acyl tXNAs containing 1',5'-anhydrohexitol (HNA), 2'-fluoro ribose (2'F-RNA) and 2'-fluoro arabinose. L-Ala-tXNA containing HNA or 2'F-RNA were substrates of the Fem enzyme. The synthesis of peptidyl-XNA and the resolution of their structures in complex with the enzyme show the impact of the XNA on protein binding. For the first time we describe functional tXNA in an in vitro assay. These results invite to test tXNA also as substitute for tRNA in translation.

PubMed: 36350642
DOI: 10.1093/nar/gkac1023

実験手法

X-RAY DIFFRACTION (1.66 Å)