7Z5T

Crystal Structure of botulinum neurotoxin A2 cell binding domain

これはPDB形式変換不可エントリーです。

7Z5T の概要

• エントリーDOI: 10.2210/pdb7z5t/pdb
• 分子名称: Botulinum neurotoxin, ACETATE ION, PENTAETHYLENE GLYCOL, ... (4 entities in total)
• 機能のキーワード: neurotoxin, botulinum, receptor, cell binding domain, toxin
• 由来する生物種: Clostridium botulinum
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 50713.59

構造登録者

Gregory, K.S.,Acharya, K.R.,Liu, S.M.,Mahadeva, T.B. (登録日: 2022-03-10, 公開日: 2022-06-08, 最終更新日: 2024-01-31)

主引用文献

Gregory, K.S.,Mahadeva, T.B.,Liu, S.M.,Acharya, K.R.
Structural Features of Clostridium botulinum Neurotoxin Subtype A2 Cell Binding Domain.
Toxins, 14:-, 2022

PubMed Abstract: Botulinum neurotoxins (BoNT) are a group of clostridial toxins that cause the potentially fatal neuroparalytic disease botulism. Although highly toxic, BoNTs are utilized as therapeutics to treat a range of neuromuscular conditions. Several serotypes (BoNT/A-/G, /X) have been identified with vastly differing toxicological profiles. Each serotype can be further sub-categorised into subtypes due to subtle variations in their protein sequence. These minor changes have been attributed to differences in both the duration of action and potency for BoNT/A subtypes. BoNTs are composed of three domains-a cell-binding domain, a translocation domain, and a catalytic domain. In this paper, we present the crystal structures of the botulinum neurotoxin A2 cell binding domain, both alone and in complex with its receptor ganglioside GD1a at 1.63 and 2.10 Å, respectively. The analysis of these structures reveals a potential redox-dependent Lys-O-Cys bridge close to the ganglioside binding site and a hinge motion between the H and H subdomains. Furthermore, we make a detailed comparison with the previously reported H/A2:SV2C structure for a comprehensive structural analysis of H/A2 receptor binding.

PubMed: 35622602
DOI: 10.3390/toxins14050356

実験手法

X-RAY DIFFRACTION (1.63 Å)