Summary for 7Z5F
| Entry DOI | 10.2210/pdb7z5f/pdb |
| EMDB information | 14521 |
| Descriptor | Capsid protein VP1 (1 entity in total) |
| Functional Keywords | capsid, mvm, vlp, virus like particle |
| Biological source | Minute virus of mice |
| Total number of polymer chains | 1 |
| Total formula weight | 64526.38 |
| Authors | Luque, D.,Ortega-Esteban, A.,Valbuena, A.,Vilas, J.L.,Rodriguez-Huete, A.,Mateu, M.G.,Caston, J.R. (deposition date: 2022-03-09, release date: 2023-03-08, Last modification date: 2024-07-17) |
| Primary citation | Luque, D.,Ortega-Esteban, A.,Valbuena, A.,Luis Vilas, J.,Rodriguez-Huete, A.,Mateu, M.G.,Caston, J.R. Equilibrium Dynamics of a Biomolecular Complex Analyzed at Single-amino Acid Resolution by Cryo-electron Microscopy. J.Mol.Biol., 435:168024-168024, 2023 Cited by PubMed Abstract: The biological function of macromolecular complexes depends not only on large-scale transitions between conformations, but also on small-scale conformational fluctuations at equilibrium. Information on the equilibrium dynamics of biomolecular complexes could, in principle, be obtained from local resolution (LR) data in cryo-electron microscopy (cryo-EM) maps. However, this possibility had not been validated by comparing, for a same biomolecular complex, LR data with quantitative information on equilibrium dynamics obtained by an established solution technique. In this study we determined the cryo-EM structure of the minute virus of mice (MVM) capsid as a model biomolecular complex. The LR values obtained correlated with crystallographic B factors and with hydrogen/deuterium exchange (HDX) rates obtained by mass spectrometry (HDX-MS), a gold standard for determining equilibrium dynamics in solution. This result validated a LR-based cryo-EM approach to investigate, with high spatial resolution, the equilibrium dynamics of biomolecular complexes. As an application of this approach, we determined the cryo-EM structure of two mutant MVM capsids and compared their equilibrium dynamics with that of the wild-type MVM capsid. The results supported a previously suggested linkage between mechanical stiffening and impaired equilibrium dynamics of a virus particle. Cryo-EM is emerging as a powerful approach for simultaneously acquiring information on the atomic structure and local equilibrium dynamics of biomolecular complexes. PubMed: 36828271DOI: 10.1016/j.jmb.2023.168024 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.22 Å) |
Structure validation
Download full validation report
