7Z4H
SpCas9 bound to 14-nucleotide complementary DNA substrate
Summary for 7Z4H
Entry DOI | 10.2210/pdb7z4h/pdb |
EMDB information | 14497 |
Descriptor | sgRNA, Target strand of 14-nucleotide complementary DNA substrate, Non-target strand of 14-nucleotide complementary DNA substrate, ... (4 entities in total) |
Functional Keywords | crispr, cas9, r-loop, substrate binding, off-target, hydrolase |
Biological source | Streptococcus pyogenes More |
Total number of polymer chains | 4 |
Total formula weight | 212469.85 |
Authors | Pacesa, M.,Jinek, M. (deposition date: 2022-03-03, release date: 2022-08-31, Last modification date: 2024-07-17) |
Primary citation | Pacesa, M.,Loeff, L.,Querques, I.,Muckenfuss, L.M.,Sawicka, M.,Jinek, M. R-loop formation and conformational activation mechanisms of Cas9. Nature, 609:191-196, 2022 Cited by PubMed: 36002571DOI: 10.1038/s41586-022-05114-0 PDB entries with the same primary citation |
Experimental method | ELECTRON MICROSCOPY (3.49 Å) |
Structure validation
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