7Z1N

Structure of yeast RNA Polymerase III Delta C53-C37-C11

7Z1N の概要

• エントリーDOI: 10.2210/pdb7z1n/pdb
• 関連するPDBエントリー: 7Z1L 7Z1M
• EMDBエントリー: 14449
• 分子名称: DNA-directed RNA polymerase III subunit RPC1, DNA-directed RNA polymerases I and III subunit RPAC2, DNA-directed RNA polymerases I, II, and III subunit RPABC4, ... (20 entities in total)
• 機能のキーワード: rna synthesis, short rnas, termination, transcription
• 由来する生物種: Saccharomyces cerevisiae W303; 詳細
• タンパク質・核酸の鎖数: 17
• 化学式量合計: 638969.64

構造登録者

Girbig, M.,Mueller, C.W. (登録日: 2022-02-24, 公開日: 2022-08-31, 最終更新日: 2024-07-17)

主引用文献

Girbig, M.,Xie, J.,Grotsch, H.,Libri, D.,Porrua, O.,Muller, C.W.
Architecture of the yeast Pol III pre-termination complex and pausing mechanism on poly(dT) termination signals.
Cell Rep, 40:111316-111316, 2022

PubMed Abstract: RNA polymerase (Pol) III is specialized to transcribe short, abundant RNAs, for which it terminates transcription on polythymine (dT) stretches on the non-template (NT) strand. When Pol III reaches the termination signal, it pauses and forms the pre-termination complex (PTC). Here, we report cryoelectron microscopy (cryo-EM) structures of the yeast Pol III PTC and complementary functional states at resolutions of 2.7-3.9 Å. Pol III recognizes the poly(dT) termination signal with subunit C128 that forms a hydrogen-bond network with the NT strand and, thereby, induces pausing. Mutating key interacting residues interferes with transcription termination in vitro, impairs yeast growth, and causes global termination defects in vivo, confirming our structural results. Additional cryo-EM analysis reveals that C53-C37, a Pol III subcomplex and key termination factor, participates indirectly in Pol III termination. We propose a mechanistic model of Pol III transcription termination and rationalize why Pol III, unlike Pol I and Pol II, terminates on poly(dT) signals.

PubMed: 36070694
DOI: 10.1016/j.celrep.2022.111316

実験手法

ELECTRON MICROSCOPY (3.9 Å)