7Z0C

Crystal structure of the K state of bacteriorhodopsin at 1.53 Angstrom resolution

7Z0C の概要

• エントリーDOI: 10.2210/pdb7z0c/pdb
• 分子名称: Bacteriorhodopsin, EICOSANE, (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate, ... (5 entities in total)
• 機能のキーワード: rhodopsin, retinal, microbial rhodopsin, ion transport, proton pump, photocycle, ultrahigh resolution, membrane protein
• 由来する生物種: Halobacterium salinarum
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 35215.05

構造登録者

Borshchevskiy, V.,Kovalev, K.,Round, E.,Efremov, R.,Bourenkov, G.,Gordeliy, V. (登録日: 2022-02-22, 公開日: 2022-05-18, 最終更新日: 2024-01-31)

主引用文献

Borshchevskiy, V.,Kovalev, K.,Round, E.,Efremov, R.,Astashkin, R.,Bourenkov, G.,Bratanov, D.,Balandin, T.,Chizhov, I.,Baeken, C.,Gushchin, I.,Kuzmin, A.,Alekseev, A.,Rogachev, A.,Willbold, D.,Engelhard, M.,Bamberg, E.,Buldt, G.,Gordeliy, V.
True-atomic-resolution insights into the structure and functional role of linear chains and low-barrier hydrogen bonds in proteins.
Nat.Struct.Mol.Biol., 29:440-450, 2022

PubMed Abstract: Hydrogen bonds are fundamental to the structure and function of biological macromolecules and have been explored in detail. The chains of hydrogen bonds (CHBs) and low-barrier hydrogen bonds (LBHBs) were proposed to play essential roles in enzyme catalysis and proton transport. However, high-resolution structural data from CHBs and LBHBs is limited. The challenge is that their 'visualization' requires ultrahigh-resolution structures of the ground and functionally important intermediate states to identify proton translocation events and perform their structural assignment. Our true-atomic-resolution structures of the light-driven proton pump bacteriorhodopsin, a model in studies of proton transport, show that CHBs and LBHBs not only serve as proton pathways, but also are indispensable for long-range communications, signaling and proton storage in proteins. The complete picture of CHBs and LBHBs discloses their multifunctional roles in providing protein functions and presents a consistent picture of proton transport and storage resolving long-standing debates and controversies.

PubMed: 35484235
DOI: 10.1038/s41594-022-00762-2

実験手法

X-RAY DIFFRACTION (1.53 Å)