7Z00
Crystal structure of Vibrio alkaline phosphatase in 1.0 M KBr
Summary for 7Z00
| Entry DOI | 10.2210/pdb7z00/pdb |
| Descriptor | Alkaline phosphatase, ZINC ION, BROMIDE ION, ... (6 entities in total) |
| Functional Keywords | alkaline phosphatase, ap, hydrolase |
| Biological source | Vibrio sp. G15-21 |
| Total number of polymer chains | 1 |
| Total formula weight | 58970.79 |
| Authors | Markusson, S.,Hjorleifsson, J.G.,Kursula, P.,Asgeirsson, B. (deposition date: 2022-02-21, release date: 2022-11-02, Last modification date: 2024-01-31) |
| Primary citation | Markusson, S.,Hjorleifsson, J.G.,Kursula, P.,Asgeirsson, B. Structural Characterization of Functionally Important Chloride Binding Sites in the Marine Vibrio Alkaline Phosphatase. Biochemistry, 61:2248-2260, 2022 Cited by PubMed Abstract: Enzyme stability and function can be affected by various environmental factors, such as temperature, pH, and ionic strength. Enzymes that are located outside the relatively unchanging environment of the cytosol, such as those residing in the periplasmic space of bacteria or extracellularly secreted, are challenged by more fluctuations in the aqueous medium. Bacterial alkaline phosphatases (APs) are generally affected by ionic strength of the medium, but this varies substantially between species. An AP from the marine bacterium (VAP) shows complex pH-dependent activation and stabilization in the 0-1.0 M range of halogen salts and has been hypothesized to specifically bind chloride anions. Here, using X-ray crystallography and anomalous scattering, we have located two chloride binding sites in the structure of VAP, one in the active site and another one at a peripheral site. Further characterization of the binding sites using site-directed mutagenesis and small-angle X-ray scattering showed that upon binding of chloride to the peripheral site, structural dynamics decreased locally, resulting in thermal stabilization of the VAP active conformation. Binding of the chloride ion in the active site did not displace the bound inorganic phosphate product, but it may promote product release by facilitating rotational stabilization of the substrate-binding Arg129. Overall, these results reveal the complex nature and dynamics of chloride binding to enzymes through long-range modulation of electronic potential in the vicinity of the active site, resulting in increased catalytic efficiency and stability. PubMed: 36194497DOI: 10.1021/acs.biochem.2c00438 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.6 Å) |
Structure validation
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