7YZY

pMMO structure from native membranes by cryoET and STA

7YZY の概要

• エントリーDOI: 10.2210/pdb7yzy/pdb
• EMDBエントリー: 14399
• 分子名称: Particulate methane monooxygenase beta subunit, Methane monooxygenase subunit C2, Particulate methane monooxygenase alpha subunit (3 entities in total)
• 機能のキーワード: pmmo, array, native membranes, membrane protein
• 由来する生物種: Methylococcus capsulatus str. Bath; 詳細
• タンパク質・核酸の鎖数: 9
• 化学式量合計: 323367.07

構造登録者

Zhu, Y.,Ni, T.,Zhang, P. (登録日: 2022-02-21, 公開日: 2022-08-10, 最終更新日: 2024-07-17)

主引用文献

Zhu, Y.,Koo, C.W.,Cassidy, C.K.,Spink, M.C.,Ni, T.,Zanetti-Domingues, L.C.,Bateman, B.,Martin-Fernandez, M.L.,Shen, J.,Sheng, Y.,Song, Y.,Yang, Z.,Rosenzweig, A.C.,Zhang, P.
Structure and activity of particulate methane monooxygenase arrays in methanotrophs.
Nat Commun, 13:5221-5221, 2022

PubMed Abstract: Methane-oxidizing bacteria play a central role in greenhouse gas mitigation and have potential applications in biomanufacturing. Their primary metabolic enzyme, particulate methane monooxygenase (pMMO), is housed in copper-induced intracytoplasmic membranes (ICMs), of which the function and biogenesis are not known. We show by serial cryo-focused ion beam (cryoFIB) milling/scanning electron microscope (SEM) volume imaging and lamellae-based cellular cryo-electron tomography (cryoET) that these ICMs are derived from the inner cell membrane. The pMMO trimer, resolved by cryoET and subtomogram averaging to 4.8 Å in the ICM, forms higher-order hexagonal arrays in intact cells. Array formation correlates with increased enzymatic activity, highlighting the importance of studying the enzyme in its native environment. These findings also demonstrate the power of cryoET to structurally characterize native membrane enzymes in the cellular context.

PubMed: 36064719
DOI: 10.1038/s41467-022-32752-9

実験手法

ELECTRON MICROSCOPY (4.8 Å)