7YYW

Molecular snapshots of drug release from tubulin: 10 nanoseconds after photoactivation.

Summary for 7YYW

• Entry DOI: 10.2210/pdb7yyw/pdb
• Descriptor: Tubulin alpha-1B chain, Tubulin beta-2B chain, Designed Ankyrin Repeat Protein (DARPIN) D1, ... (9 entities in total)
• Functional Keywords: time-resolved serial crystallography, tubulin dynamics, azobenzene dynamics, cell cycle
• Biological source: synthetic construct; More
• Total number of polymer chains: 3
• Total formula weight: 119621.86

Authors

Wranik, M.,Weinert, T.,Standfuss, J. (deposition date: 2022-02-18, release date: 2023-02-22, Last modification date: 2023-09-27)

Primary citation

Wranik, M.,Weinert, T.,Slavov, C.,Masini, T.,Furrer, A.,Gaillard, N.,Gioia, D.,Ferrarotti, M.,James, D.,Glover, H.,Carrillo, M.,Kekilli, D.,Stipp, R.,Skopintsev, P.,Brunle, S.,Muhlethaler, T.,Beale, J.,Gashi, D.,Nass, K.,Ozerov, D.,Johnson, P.J.M.,Cirelli, C.,Bacellar, C.,Braun, M.,Wang, M.,Dworkowski, F.,Milne, C.,Cavalli, A.,Wachtveitl, J.,Steinmetz, M.O.,Standfuss, J.
Watching the release of a photopharmacological drug from tubulin using time-resolved serial crystallography.
Nat Commun, 14:903-903, 2023

PubMed Abstract: The binding and release of ligands from their protein targets is central to fundamental biological processes as well as to drug discovery. Photopharmacology introduces chemical triggers that allow the changing of ligand affinities and thus biological activity by light. Insight into the molecular mechanisms of photopharmacology is largely missing because the relevant transitions during the light-triggered reaction cannot be resolved by conventional structural biology. Using time-resolved serial crystallography at a synchrotron and X-ray free-electron laser, we capture the release of the anti-cancer compound azo-combretastatin A4 and the resulting conformational changes in tubulin. Nine structural snapshots from 1 ns to 100 ms complemented by simulations show how cis-to-trans isomerization of the azobenzene bond leads to a switch in ligand affinity, opening of an exit channel, and collapse of the binding pocket upon ligand release. The resulting global backbone rearrangements are related to the action mechanism of microtubule-destabilizing drugs.

PubMed: 36807348
DOI: 10.1038/s41467-023-36481-5

Experimental method

X-RAY DIFFRACTION (2.2 Å)