7YWW

Monocot chimeric jacalin JAC1 from Oryza sativa: lectin domain (crystal form 2)

7YWW の概要

• エントリーDOI: 10.2210/pdb7yww/pdb
• 関連するPDBエントリー: 7R5Z 7YWE 7YWF 7YWG
• 分子名称: Dirigent protein, IODIDE ION, PENTAETHYLENE GLYCOL, ... (5 entities in total)
• 機能のキーワード: monocot chimeric jacalin, dirigent protein, lectin, pathogen resistance, plant protein
• 由来する生物種: Oryza sativa (rice)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 39982.89

構造登録者

Huwa, N.,Classen, T.,Weiergraeber, O.H. (登録日: 2022-02-14, 公開日: 2022-05-04, 最終更新日: 2024-01-31)

主引用文献

Huwa, N.,Weiergraber, O.H.,Fejzagic, A.V.,Kirsch, C.,Schaffrath, U.,Classen, T.
The Crystal Structure of the Defense Conferring Rice Protein Os JAC1 Reveals a Carbohydrate Binding Site on the Dirigent-like Domain.
Biomolecules, 12:-, 2022

PubMed Abstract: Pesticides are routinely used to prevent severe losses in agriculture. This practice is under debate because of its potential negative environmental impact and selection of resistances in pathogens. Therefore, the development of disease resistant plants is mandatory. It was shown that the rice () protein JAC1 enhances resistance against different bacterial and fungal plant pathogens in rice, barley, and wheat. Recently we reported possible carbohydrate interaction partners for both domains of JAC1 (a jacalin-related lectin (JRL) and a dirigent (DIR) domain), however, a mechanistic understanding of its function is still lacking. Here, we report crystal structures for both individual domains and the complex of galactobiose with the DIR domain, which revealed a new carbohydrate binding motif for DIR proteins. Docking studies of the two domains led to a model of the full-length protein. Our findings offer insights into structure and binding properties of JAC1 and its possible function in pathogen resistance.

PubMed: 36009020
DOI: 10.3390/biom12081126

実験手法

X-RAY DIFFRACTION (1.4 Å)