7YV5

genetically encoded pH sensor Lime at pH6

Summary for 7YV5

• Entry DOI: 10.2210/pdb7yv5/pdb
• Descriptor: Lime (1 entity in total)
• Functional Keywords: ph sensor, genetically encoded, florescent protein, fluorescent protein
• Biological source: Aequorea victoria
• Total number of polymer chains: 1
• Total formula weight: 26812.08

Authors

Wen, Y.,Shen, Y.,Campbell, R.,Lemieux, M.J. (deposition date: 2022-08-18, release date: 2023-08-16, Last modification date: 2024-11-06)

Primary citation

Shen, Y.,Wen, Y.,Sposini, S.,Vishwanath, A.A.,Abdelfattah, A.S.,Schreiter, E.R.,Lemieux, M.J.,de Juan-Sanz, J.,Perrais, D.,Campbell, R.E.
Rational Engineering of an Improved Genetically Encoded pH Sensor Based on Superecliptic pHluorin.
ACS Sens, 8:3014-3022, 2023

PubMed Abstract: Genetically encoded pH sensors based on fluorescent proteins are valuable tools for the imaging of cellular events that are associated with pH changes, such as exocytosis and endocytosis. Superecliptic pHluorin (SEP) is a pH-sensitive green fluorescent protein (GFP) variant widely used for such applications. Here, we report the rational design, development, structure, and applications of Lime, an improved SEP variant with higher fluorescence brightness and greater pH sensitivity. The X-ray crystal structure of Lime supports the mechanistic rationale that guided the introduction of beneficial mutations. Lime provides substantial improvements relative to SEP for imaging of endocytosis and exocytosis. Furthermore, Lime and its variants are advantageous for a broader range of applications including the detection of synaptic release and neuronal voltage changes.

PubMed: 37481776
DOI: 10.1021/acssensors.3c00484

Experimental method

X-RAY DIFFRACTION (2.85 Å)