7YRA

Crystal structure of [2Fe-2S]-TtPetA

7YRA の概要

• エントリーDOI: 10.2210/pdb7yra/pdb
• 関連するPDBエントリー: 7YR9
• 分子名称: Ubiquinol-cytochrome c reductase iron-sulfur subunit, FE2/S2 (INORGANIC) CLUSTER, SULFATE ION, ... (6 entities in total)
• 機能のキーワード: rieske, iron-sulfur cluster, metal binding, electron transport
• 由来する生物種: Thermochromatium tepidum
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 33947.40

構造登録者

Tsutsumi, E.,Niwa, S.,Takeda, K. (登録日: 2022-08-09, 公開日: 2023-09-20, 最終更新日: 2024-10-23)

主引用文献

Tsutsumi, E.,Niwa, S.,Takeda, R.,Sakamoto, N.,Okatsu, K.,Fukai, S.,Ago, H.,Nagao, S.,Sekiguchi, H.,Takeda, K.
Structure of a putative immature form of a Rieske-type iron-sulfur protein in complex with zinc chloride.
Commun Chem, 6:190-190, 2023

PubMed Abstract: Iron-sulfur clusters are prosthetic groups of proteins involved in various biological processes. However, details of the immature state of the iron-sulfur cluster into proteins have not yet been elucidated. We report here the first structural analysis of the Zn-containing form of a Rieske-type iron-sulfur protein, PetA, from Thermochromatium tepidum (TtPetA) by X-ray crystallography and small-angle X-ray scattering analysis. The Zn-containing form of TtPetA was indicated to be a dimer in solution. The zinc ion adopts a regular tetra-coordination with two chloride ions and two cysteine residues. Only a histidine residue in the cluster-binding site exhibited a conformational difference from the [2Fe-2S] containing form. The Zn-containing structure indicates that the conformation of the cluster binding site is already constructed and stabilized before insertion of [2Fe-2S]. The binding mode of ZnCl, similar to the [2Fe-2S] cluster, suggests that the zinc ions might be involved in the insertion of the [2Fe-2S] cluster.

PubMed: 37689761
DOI: 10.1038/s42004-023-01000-6

実験手法

X-RAY DIFFRACTION (1.79 Å)