7YQD

EM structure of human PA28gamma (wild-type)

7YQD の概要

• エントリーDOI: 10.2210/pdb7yqd/pdb
• EMDBエントリー: 34024
• 分子名称: Proteasome activator complex subunit 3 (1 entity in total)
• 機能のキーワード: proteasome activator gamma, pa28gamma, psme3, protein transport
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 206829.50

構造登録者

Chen, D.-D.,Hao, J.,Yun, C.-H. (登録日: 2022-08-06, 公開日: 2022-09-21, 最終更新日: 2025-06-18)

主引用文献

Chen, D.D.,Hao, J.,Shen, C.H.,Deng, X.M.,Yun, C.H.
Atomic resolution Cryo-EM structure of human proteasome activator PA28 gamma.
Int.J.Biol.Macromol., 219:500-507, 2022

PubMed Abstract: The PA28 family proteasome activators play important roles in regulating proteasome activities. Though the three paralogs (PA28α, PA28β, and PA28γ) are similar in terms of primary sequence, they show significant differences in expression pattern, cellular localization and most importantly, biological functions. While PA28αβ is responsible for promoting peptidase activity of proteasome to facilitate MHC-I antigen processing, but unable to promote protein degradation, PA28γ is well-known to not only promote peptidase activity but also proteolytic activity of proteasome. However, why this paralog has the unique function remains elusive. Previous structural studies have mainly focused on mammalian PA28α, PA28β and PA28αβ heptamers, while structural studies on mammalian PA28γ of atomic resolution are still absent to date. In the present work, we determined the Cryo-EM structure of the human PA28γ heptamer at atomic resolution, revealing interesting unique structural features that may hint our understanding the functional mechanisms of this proteasome activator.

PubMed: 35932807
DOI: 10.1016/j.ijbiomac.2022.07.246

実験手法

ELECTRON MICROSCOPY (3.4 Å)