7YP9

Cryo-EM structure of Escherichia coli paused complex of transcription termination (TTC-pause)

7YP9 の概要

• エントリーDOI: 10.2210/pdb7yp9/pdb
• EMDBエントリー: 33996
• 分子名称: DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... (9 entities in total)
• 機能のキーワード: termination, intrinsic termination, u-tract pause, transcription pause, factor-independent termination, transcription-dna-rna complex, transcription/dna/rna
• 由来する生物種: Escherichia coli K-12; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 416394.07

構造登録者

You, L.L.,Zhang, Y. (登録日: 2022-08-03, 公開日: 2022-11-16, 最終更新日: 2024-07-03)

主引用文献

You, L.,Omollo, E.O.,Yu, C.,Mooney, R.A.,Shi, J.,Shen, L.,Wu, X.,Wen, A.,He, D.,Zeng, Y.,Feng, Y.,Landick, R.,Zhang, Y.
Structural basis for intrinsic transcription termination.
Nature, 613:783-789, 2023

PubMed Abstract: Efficient and accurate termination is required for gene transcription in all living organisms. Cellular RNA polymerases in both bacteria and eukaryotes can terminate their transcription through a factor-independent termination pathway-called intrinsic termination transcription in bacteria-in which RNA polymerase recognizes terminator sequences, stops nucleotide addition and releases nascent RNA spontaneously. Here we report a set of single-particle cryo-electron microscopy structures of Escherichia coli transcription intrinsic termination complexes representing key intermediate states of the event. The structures show how RNA polymerase pauses at terminator sequences, how the terminator RNA hairpin folds inside RNA polymerase, and how RNA polymerase rewinds the transcription bubble to release RNA and then DNA. These macromolecular snapshots define a structural mechanism for bacterial intrinsic termination and a pathway for RNA release and DNA collapse that is relevant for factor-independent termination by all RNA polymerases.

PubMed: 36631609
DOI: 10.1038/s41586-022-05604-1

実験手法

ELECTRON MICROSCOPY (3.58 Å)