7YOZ

Cryo-EM structure of human subnucleosome (intermediate form)

7YOZ の概要

• エントリーDOI: 10.2210/pdb7yoz/pdb
• EMDBエントリー: 33991
• 分子名称: Histone H3.1, Histone H4, Widom601 DNA FW (145-MER), ... (4 entities in total)
• 機能のキーワード: subnucleosome, nuclear protein, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 199096.64

構造登録者

Nozawa, K.,Takizawa, Y.,Kurumizaka, H. (登録日: 2022-08-02, 公開日: 2022-11-16, 最終更新日: 2024-07-03)

主引用文献

Nozawa, K.,Takizawa, Y.,Pierrakeas, L.,Sogawa-Fujiwara, C.,Saikusa, K.,Akashi, S.,Luk, E.,Kurumizaka, H.
Cryo-electron microscopy structure of the H3-H4 octasome: A nucleosome-like particle without histones H2A and H2B.
Proc.Natl.Acad.Sci.USA, 119:e2206542119-e2206542119, 2022

PubMed Abstract: The canonical nucleosome, which represents the major packaging unit of eukaryotic chromatin, has an octameric core composed of two histone H2A-H2B and H3-H4 dimers with ∼147 base pairs (bp) of DNA wrapped around it. Non-nucleosomal particles with alternative histone stoichiometries and DNA wrapping configurations have been found, and they could profoundly influence genome architecture and function. Using cryo-electron microscopy, we solved the structure of the H3-H4 octasome, a nucleosome-like particle with a di-tetrameric core consisting exclusively of the H3 and H4 histones. The core is wrapped by ∼120 bp of DNA in 1.5 negative superhelical turns, forming two stacked disks that are connected by a H4-H4' four-helix bundle. Three conformations corresponding to alternative interdisk angles were observed, indicating the flexibility of the H3-H4 octasome structure. In vivo crosslinking experiments detected histone-histone interactions consistent with the H3-H4 octasome model, suggesting that H3-H4 octasomes or related structural features exist in cells.

PubMed: 36322721
DOI: 10.1073/pnas.2206542119

実験手法

ELECTRON MICROSCOPY (4.3 Å)