7YLK

Myoglobin containing Ir complex

7YLK の概要

• エントリーDOI: 10.2210/pdb7ylk/pdb
• 分子名称: Myoglobin, delta-{1-([2,2'-bipyridin]-5-ylmethyl)pyrrolidine-2,5-dione}bis[2-(2,4-difluorophenyl)pyridine)]iridium(III), lambda-{1-([2,2'-bipyridin]-5-ylmethyl)pyrrolidine-2,5-dione}bis[2-(2,4-difluorophenyl)pyridine)]iridium(III), ... (5 entities in total)
• 機能のキーワード: artificial metallocofactor, oxygen storage
• 由来する生物種: Physeter catodon (sperm whale)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 18881.54

構造登録者

Lee, J.H.,Song, W.J. (登録日: 2022-07-26, 公開日: 2023-03-15, 最終更新日: 2023-11-29)

主引用文献

Lee, J.,Song, W.J.
Photocatalytic C-O Coupling Enzymes That Operate via Intramolecular Electron Transfer.
J.Am.Chem.Soc., 145:5211-5221, 2023

PubMed Abstract: Efficient and environmentally friendly conversion of light energy for direct utilization in chemical production has been a long-standing goal in enzyme design. Herein, we synthesized artificial photocatalytic enzymes by introducing an Ir photocatalyst and a Ni(bpy) complex to an optimal protein scaffold in close proximity. Consequently, the enzyme generated C-O coupling products with up to 96% yields by harvesting visible light and performing intramolecular electron transfer between the two catalysts. We systematically modulated the catalytic activities of the artificial photocatalytic cross-coupling enzymes by tuning the electrochemical properties of the catalytic components, their positions, and distances within a protein. As a result, we discovered the best-performing mutant that showed broad substrate scopes under optimized conditions. This work explicitly demonstrated that we could integrate and control both the inorganic and biochemical components of photocatalytic biocatalysis to achieve high yield and selectivity in valuable chemical transformations.

PubMed: 36825656
DOI: 10.1021/jacs.2c12226

実験手法

X-RAY DIFFRACTION (1.63 Å)