7YHR

Anti-CRISPR protein AcrIC5

Summary for 7YHR

• Entry DOI: 10.2210/pdb7yhr/pdb
• Descriptor: Anti-CRISPR protein Type I-C5 (2 entities in total)
• Functional Keywords: acric5, immune system, inhibitor, anti-crispr
• Biological source: Pseudomonas delhiensis
• Total number of polymer chains: 1
• Total formula weight: 6821.37

Authors

Kang, Y.J.,Park, H.H. (deposition date: 2022-07-14, release date: 2023-05-24, Last modification date: 2024-06-05)

Primary citation

Kang, Y.J.,Park, H.H.
High-resolution crystal structure of the anti-CRISPR protein AcrIC5.
Biochem.Biophys.Res.Commun., 625:102-108, 2022

PubMed Abstract: As a result of the long-term battle of bacteria and archaea against invaders such as viruses and genetic mobile elements, they have developed CRISPR-Cas systems for self-defense, which allows them to remove the viral genetic material introduced into host cells via infection. To fight against this bacterial immune system, however, viruses have also evolved to produce multiple anti-CRISPR proteins that can inhibit the bacterial CRISPR-Cas system. In this study, we introduced a tentative inhibitory activity against a type I-C CRISPR-Cas system by determining the crystal structure of AcrIC5 from Pseudomonas delhiensis. Structural analysis revealed that AcrIC5 was composed of noble folds comprising two antiparallel sheets and three helices. Although AcrIC5 did not directly interact with either the type I-C cascade from Neisseria lactamia or the type I-F cascade from Pseudomonas aeruginosa in our analysis, a highly acidic surface feature indicated that AcrIC5 may be DNA mimic Acrs that directly binds to the target DNA binding site in type I-C cascade and inhibits the recruitment of the target DNA to this cascade.

PubMed: 35952606
DOI: 10.1016/j.bbrc.2022.08.005

Experimental method

X-RAY DIFFRACTION (1.45 Å)