7YG6

Cryo-EM structure of the EfPiwi(N959K) in complex with piRNA

7YG6 の概要

• エントリーDOI: 10.2210/pdb7yg6/pdb
• EMDBエントリー: 33809
• 分子名称: piRNA, Piwi, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: pirna, piwi protein, argonaute, rna binding protein, rna binding protein-rna complex, rna binding protein/rna
• 由来する生物種: Ephydatia fluviatilis; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 100660.50

構造登録者

Li, Z.Q.,Liu, H.B.,Wu, J.P.,Shen, E.Z. (登録日: 2022-07-11, 公開日: 2024-01-24, 最終更新日: 2024-05-08)

主引用文献

Li, Z.,Li, Z.,Zhang, Y.,Zhou, L.,Xu, Q.,Li, L.,Zeng, L.,Xue, J.,Niu, H.,Zhong, J.,Yu, Q.,Li, D.,Gui, M.,Huang, Y.,Tu, S.,Zhang, Z.,Song, C.Q.,Wu, J.,Shen, E.Z.
Mammalian PIWI-piRNA-target complexes reveal features for broad and efficient target silencing.
Nat.Struct.Mol.Biol., 2024

PubMed Abstract: The PIWI-interacting RNA (piRNA) pathway is an adaptive defense system wherein piRNAs guide PIWI family Argonaute proteins to recognize and silence ever-evolving selfish genetic elements and ensure genome integrity. Driven by this intensive host-pathogen arms race, the piRNA pathway and its targeted transposons have coevolved rapidly in a species-specific manner, but how the piRNA pathway adapts specifically to target silencing in mammals remains elusive. Here, we show that mouse MILI and human HILI piRNA-induced silencing complexes (piRISCs) bind and cleave targets more efficiently than their invertebrate counterparts from the sponge Ephydatia fluviatilis. The inherent functional differences comport with structural features identified by cryo-EM studies of piRISCs. In the absence of target, MILI and HILI piRISCs adopt a wider nucleic-acid-binding channel and display an extended prearranged piRNA seed as compared with EfPiwi piRISC, consistent with their ability to capture targets more efficiently than EfPiwi piRISC. In the presence of target, the seed gate-which enforces seed-target fidelity in microRNA RISC-adopts a relaxed state in mammalian piRISC, revealing how MILI and HILI tolerate seed-target mismatches to broaden the target spectrum. A vertebrate-specific lysine distorts the piRNA seed, shifting the trajectory of the piRNA-target duplex out of the central cleft and toward the PAZ lobe. Functional analyses reveal that this lysine promotes target binding and cleavage. Our study therefore provides a molecular basis for the piRNA targeting mechanism in mice and humans, and suggests that mammalian piRNA machinery can achieve broad target silencing using a limited supply of piRNA species.

PubMed: 38658622
DOI: 10.1038/s41594-024-01287-6

実験手法

ELECTRON MICROSCOPY (3.2 Å)