7YC8

Cryo-EM structure of Tetrahymena ribozyme conformation 1 undergoing the first-step self-splicing

7YC8 の概要

• エントリーDOI: 10.2210/pdb7yc8/pdb
• EMDBエントリー: 33736
• 分子名称: RNA (388-MER), GUANOSINE-5'-TRIPHOSPHATE (2 entities in total)
• 機能のキーワード: tetrahymena ribozyme, first step of self-splicing, conformation 1, rna
• 由来する生物種: Tetrahymena
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 127535.06

構造登録者

Zhang, X.,Li, S.,Pintilie, G.,Palo, M.Z.,Zhang, K. (登録日: 2022-07-01, 公開日: 2023-07-05, 最終更新日: 2023-08-16)

主引用文献

Zhang, X.,Li, S.,Pintilie, G.,Palo, M.Z.,Zhang, K.
Snapshots of the first-step self-splicing of Tetrahymena ribozyme revealed by cryo-EM.
Nucleic Acids Res., 51:1317-1325, 2023

PubMed Abstract: Tetrahymena ribozyme is a group I intron, whose self-splicing is the result of two sequential ester-transfer reactions. To understand how it facilitates catalysis in the first self-splicing reaction, we used cryogenic electron microscopy (cryo-EM) to resolve the structures of L-16 Tetrahymena ribozyme complexed with a 11-nucleotide 5'-splice site analog substrate. Four conformations were achieved to 4.14, 3.18, 3.09 and 2.98 Å resolutions, respectively, corresponding to different splicing intermediates during the first enzymatic reaction. Comparison of these structures reveals structural alterations, including large conformational changes in IGS/IGSext (P1-P1ext duplex) and J5/4, as well as subtle local rearrangements in the G-binding site. These structural changes are required for the enzymatic activity of the Tetrahymena ribozyme. Our study demonstrates the ability of cryo-EM to capture dynamic RNA structural changes, ushering in a new era in the analysis of RNA structure-function by cryo-EM.

PubMed: 36660826
DOI: 10.1093/nar/gkac1268

実験手法

ELECTRON MICROSCOPY (4.14 Å)