7Y8V

Crystal structure of AlbEF homolog mutant (AlbF-H54A/H58A) from Quasibacillus thermotolerans

7Y8V の概要

• エントリーDOI: 10.2210/pdb7y8v/pdb
• 関連するPDBエントリー: 7Y8U 7Y8X
• 分子名称: AlbE homolog, AlbF homolog H54A/H58A mutant, PHOSPHATE ION, ... (5 entities in total)
• 機能のキーワード: subtilosin biosynthesis, m16b metalloprotease, macrocyclase, hydrolase
• 由来する生物種: Quasibacillus thermotolerans; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 89349.46

構造登録者

Ishida, K.,Nakamura, A.,Kojima, S. (登録日: 2022-06-24, 公開日: 2022-10-12, 最終更新日: 2023-11-29)

主引用文献

Ishida, K.,Nakamura, A.,Kojima, S.
Crystal structure of the AlbEF complex involved in subtilosin A biosynthesis.
Structure, 30:1637-1646.e3, 2022

PubMed Abstract: Subtilosin A is a sactipeptide bacteriocin produced by Bacillus subtilis strain 168, containing intramolecular thioether bonds and a head-to-tail macrocyclic peptide bond. Macrocyclization is presumably catalyzed by AlbE and AlbF proteins encoded by the subtilosin A biosynthesis gene cluster. However, the underlying mechanism of macrocyclization remains uncertain as the tertiary structures of the proteins are undetermined. Here, we report the crystal structures of AlbE and AlbF homologs in Quasibacillus thermotolerans, wherein the subtilosin biosynthesis gene cluster is highly conserved. Structural analysis and pull-down assays revealed that AlbE and AlbF form heterodimeric complexes. Although the AlbEF complex shows structural similarity to M16B family metalloproteases, the substrate-binding chamber is shallower and more open than the other M16B family proteins. The chamber surface showed electrostatic complementarity to the precursor of subtilosin. Our findings provide insights into the role of AlbEF in metalloprotease catalysis and macrocyclic peptide bond formation.

PubMed: 36302388
DOI: 10.1016/j.str.2022.10.002

実験手法

X-RAY DIFFRACTION (2.3 Å)