7Y87

class I diterpene synthase mutant (CyS-C59A) from Streptomyces cattleya

7Y87 の概要

• エントリーDOI: 10.2210/pdb7y87/pdb
• 分子名称: Putative glutamate dehydrogenase/leucine dehydrogenase (2 entities in total)
• 機能のキーワード: class i diterpene synthase mutant (cys-c59a) from streptomyces cattleya, biosynthetic protein
• 由来する生物種: Streptomyces cattleya
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 42018.49

構造登録者

Xing, B.,Ma, M. (登録日: 2022-06-23, 公開日: 2022-12-21, 最終更新日: 2023-11-29)

主引用文献

Xing, B.,Xu, H.,Li, A.,Lou, T.,Xu, M.,Wang, K.,Xu, Z.,Dickschat, J.S.,Yang, D.,Ma, M.
Crystal Structure Based Mutagenesis of Cattleyene Synthase Leads to the Generation of Rearranged Polycyclic Diterpenes.
Angew.Chem.Int.Ed.Engl., 61:e202209785-e202209785, 2022

PubMed Abstract: The crystal structures of cattleyene synthase (apo-CyS), and CyS complexed with geranylgeranyl pyrophosphate (GGPP) were solved. The CyS variant exhibited an increased production of cattleyene and other diterpenes with diverse skeletons. Its structure showed a widened active site cavity explaining the relaxed selectivity. Isotopic labeling experiments revealed a remarkable cyclization mechanism involving several skeletal rearrangements for one of the novel diterpenes.

PubMed: 35819825
DOI: 10.1002/anie.202209785

実験手法

X-RAY DIFFRACTION (2.3 Å)