7Y6E

Crystal structure of sDscam FNIII23 domains, isoform Beta2v6

7Y6E の概要

• エントリーDOI: 10.2210/pdb7y6e/pdb
• 分子名称: Dscam (2 entities in total)
• 機能のキーワード: cell surface receptor, cell adhesion
• 由来する生物種: Chelicerata
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 156549.61

構造登録者

Chen, Q.,Yu, Y.,Cheng, J. (登録日: 2022-06-20, 公開日: 2023-05-24, 最終更新日: 2024-05-29)

主引用文献

Cheng, J.,Yu, Y.,Wang, X.,Zheng, X.,Liu, T.,Hu, D.,Jin, Y.,Lai, Y.,Fu, T.M.,Chen, Q.
Structural basis for the self-recognition of sDSCAM in Chelicerata.
Nat Commun, 14:2522-2522, 2023

PubMed Abstract: To create a functional neural circuit, neurons develop a molecular identity to discriminate self from non-self. The invertebrate Dscam family and vertebrate Pcdh family are implicated in determining synaptic specificity. Recently identified in Chelicerata, a shortened Dscam (sDscam) has been shown to resemble the isoform-generating characters of both Dscam and Pcdh and represent an evolutionary transition. Here we presented the molecular details of sDscam self-recognition via both trans and cis interactions using X-ray crystallographic data and functional assays. Based on our results, we proposed a molecular zipper model for the assemblies of sDscam to mediate cell-cell recognition. In this model, sDscam utilized FNIII domain to form side-by-side interactions with neighboring molecules in the same cell while established hand-in-hand interactions via Ig1 domain with molecules from another cell around. Together, our study provided a framework for understanding the assembly, recognition, and evolution of sDscam.

PubMed: 37130844
DOI: 10.1038/s41467-023-38205-1

実験手法

X-RAY DIFFRACTION (3.034 Å)