7Y49

Cryo-EM Structure of apo mitochondrial ABC transporter ABCB10 from Biortus

7Y49 の概要

• エントリーDOI: 10.2210/pdb7y49/pdb
• 関連するPDBエントリー: 4AYX
• EMDBエントリー: 33604
• 分子名称: ATP-binding cassette sub-family B member 10, mitochondrial (1 entity in total)
• 機能のキーワード: abcb10, abc transporter, biliverdin, membrane protein
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 67180.13

構造登録者

Cao, S.,Yang, Y. (登録日: 2022-06-14, 公開日: 2023-04-19, 最終更新日: 2024-04-10)

主引用文献

Cao, S.,Yang, Y.,He, L.,Hang, Y.,Yan, X.,Shi, H.,Wu, J.,Ouyang, Z.
Cryo-EM structures of mitochondrial ABC transporter ABCB10 in apo and biliverdin-bound form.
Nat Commun, 14:2030-2030, 2023

PubMed Abstract: ABCB10, a member of ABC transporter superfamily that locates in the inner membrane of mitochondria, plays crucial roles in hemoglobin synthesis, antioxidative stress and stabilization of the iron transporter mitoferrin-1. Recently, it was found that ABCB10 is a mitochondrial biliverdin exporter. However, the molecular mechanism of biliverdin export by ABCB10 remains elusive. Here we report the cryo-EM structures of ABCB10 in apo (ABCB10-apo) and biliverdin-bound form (ABCB10-BV) at 3.67 Å and 2.85 Å resolution, respectively. ABCB10-apo adopts a wide-open conformation and may thus represent the apo form structure. ABCB10-BV forms a closed conformation and biliverdin situates in a hydrophobic pocket in one protomer and bridges the interaction through hydrogen bonds with the opposing one. We also identify cholesterols sandwiched by BVs and discuss the export dynamics based on these structural and biochemical observations.

PubMed: 37041204
DOI: 10.1038/s41467-023-37851-9

実験手法

ELECTRON MICROSCOPY (3.67 Å)