7Y44

Re-refinement of damage free X-ray structure of bovine cytochrome c oxidase at 1.9 angstrom resolution

7Y44 の概要

• エントリーDOI: 10.2210/pdb7y44/pdb
• 分子名称: Cytochrome c oxidase subunit 1, Cytochrome c oxidase subunit 7A1, Cytochrome c oxidase subunit 7B, ... (30 entities in total)
• 機能のキーワード: respiratory enzyme, membrane protein, heme protein, oxidoreductase
• 由来する生物種: Bos taurus (cattle); 詳細
• タンパク質・核酸の鎖数: 26
• 化学式量合計: 456491.64

構造登録者

Tsukihara, T.,Hirata, K.,Ago, H. (登録日: 2022-06-14, 公開日: 2022-07-20, 最終更新日: 2024-05-01)

主引用文献

Hirata, K.,Shinzawa-Itoh, K.,Yano, N.,Takemura, S.,Kato, K.,Hatanaka, M.,Muramoto, K.,Kawahara, T.,Tsukihara, T.,Yamashita, E.,Tono, K.,Ueno, G.,Hikima, T.,Murakami, H.,Inubushi, Y.,Yabashi, M.,Ishikawa, T.,Yamamoto, M.,Ogura, T.,Sugimoto, H.,Shen, J.R.,Yoshikawa, S.,Ago, H.
Determination of damage-free crystal structure of an X-ray-sensitive protein using an XFEL.
Nat.Methods, 11:734-736, 2014

PubMed Abstract: We report a method of femtosecond crystallography for solving radiation damage-free crystal structures of large proteins at sub-angstrom spatial resolution, using a large single crystal and the femtosecond pulses of an X-ray free-electron laser (XFEL). We demonstrated the performance of the method by determining a 1.9-Å radiation damage-free structure of bovine cytochrome c oxidase, a large (420-kDa), highly radiation-sensitive membrane protein.

PubMed: 24813624
DOI: 10.1038/nmeth.2962

実験手法

X-RAY DIFFRACTION (1.9 Å)