7Y3F

Structure of the Anabaena PSI-monomer-IsiA supercomplex

7Y3F の概要

• エントリーDOI: 10.2210/pdb7y3f/pdb
• EMDBエントリー: 33593
• 分子名称: Photosystem I P700 chlorophyll a apoprotein A1, PsaM, Photosystem I 4.8 kDa protein, ... (23 entities in total)
• 機能のキーワード: photosystem i, electron transport, isia, photosynthesis
• 由来する生物種: Nostoc sp.; 詳細
• タンパク質・核酸の鎖数: 17
• 化学式量合計: 617159.68

構造登録者

Nagao, R.,Kato, K.,Hamaguchi, T.,Kawakami, K.,Yonekura, K.,Shen, J.R. (登録日: 2022-06-10, 公開日: 2023-03-01, 最終更新日: 2024-10-23)

主引用文献

Nagao, R.,Kato, K.,Hamaguchi, T.,Ueno, Y.,Tsuboshita, N.,Shimizu, S.,Furutani, M.,Ehira, S.,Nakajima, Y.,Kawakami, K.,Suzuki, T.,Dohmae, N.,Akimoto, S.,Yonekura, K.,Shen, J.R.
Structure of a monomeric photosystem I core associated with iron-stress-induced-A proteins from Anabaena sp. PCC 7120.
Nat Commun, 14:920-920, 2023

PubMed Abstract: Iron-stress-induced-A proteins (IsiAs) are expressed in cyanobacteria under iron-deficient conditions. The cyanobacterium Anabaena sp. PCC 7120 has four isiA genes; however, their binding property and functional roles in PSI are still missing. We analyzed a cryo-electron microscopy structure of a PSI-IsiA supercomplex isolated from Anabaena grown under an iron-deficient condition. The PSI-IsiA structure contains six IsiA subunits associated with the PsaA side of a PSI core monomer. Three of the six IsiA subunits were identified as IsiA1 and IsiA2. The PSI-IsiA structure lacks a PsaL subunit; instead, a C-terminal domain of IsiA2 occupies the position of PsaL, which inhibits the oligomerization of PSI, leading to the formation of a PSI monomer. Furthermore, excitation-energy transfer from IsiAs to PSI appeared with a time constant of 55 ps. These findings provide insights into both the molecular assembly of the Anabaena IsiA family and the functional roles of IsiAs.

PubMed: 36805598
DOI: 10.1038/s41467-023-36504-1

実験手法

ELECTRON MICROSCOPY (2.62 Å)