7XXJ

Echo 18 incubated with FcRn at pH5.5

7XXJ の概要

• エントリーDOI: 10.2210/pdb7xxj/pdb
• EMDBエントリー: 33499 33502 33505
• 分子名称: VP1, VP2, VP3, ... (5 entities in total)
• 機能のキーワード: echo 18, ph5.5, rcrn, virus
• 由来する生物種: Echovirus E18; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 94145.21

構造登録者

Liu, C.C.,Qu, X. (登録日: 2022-05-30, 公開日: 2022-06-29, 最終更新日: 2024-07-03)

主引用文献

Chen, X.,Qu, X.,Liu, C.,Zhang, Y.,Zhang, G.,Han, P.,Duan, Y.,Li, Q.,Wang, L.,Ruan, W.,Wang, P.,Wei, W.,Gao, G.F.,Zhao, X.,Xie, Z.
Human FcRn Is a Two-in-One Attachment-Uncoating Receptor for Echovirus 18.
Mbio, 13:e0116622-e0116622, 2022

PubMed Abstract: Virus-receptor interactions determine viral host range and tissue tropism. CD55 and human neonatal Fc receptor (FcRn) were found to be the binding and uncoating receptors for some of the echovirus-related enterovirus species B serotypes in our previous study. Echovirus 18 (E18), as a member of enterovirus species B, is a significant causative agent of aseptic meningitis and viral encephalitis in children. However, it does not use CD55 as a critical host factor. We conducted CRISPR/Cas9 knockout screening to determine the receptors and entry mechanisms and identified FcRn working as a dual-function receptor for E18. Knockout of and , which encode the two subunits of FcRn, prevented infection by E18 and other echoviruses in the same physiological cluster. We then elucidated the underlying molecular mechanism of receptor recognition by E18 using cryogenic electron microscopy. The binding of the FCGRT subunit to the canyon region rotates the residues around the pocket, triggering the release of the pocket factor as observed for other enterovirus species B members. E18 is a member of enterovirus species B. As one of the most common enterovirus serotypes in nonpolio enterovirus detection, it easily infects children and causes various clinical symptoms. Aseptic meningitis and viral encephalitis are the most commonly reported syndromes associated with E18. No effective antiviral drugs or approved vaccines are available. Previous studies showed that CD55 and FcRn were the binding and uncoating receptors for some echoviruses. However, we found that CD55 is not the critical host factor for E18. Thus, we want to determine the receptors and elucidate the entry mechanism of E18. Our findings reveal that FcRn is a two-in-one attachment-uncoating receptor for E18.

PubMed: 35862785
DOI: 10.1128/mbio.01166-22

実験手法

ELECTRON MICROSCOPY (3.33 Å)