7XX4

designed glycosyltransferase

7XX4 の概要

• エントリーDOI: 10.2210/pdb7xx4/pdb
• 分子名称: Oleandomycin glycosyltransferase, URIDINE-5'-DIPHOSPHATE-GLUCOSE (3 entities in total)
• 機能のキーワード: glycosyltransferase, transferase
• 由来する生物種: Streptomyces antibioticus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 90691.78

構造登録者

Lu, M.,Wu, X. (登録日: 2022-05-28, 公開日: 2023-06-14, 最終更新日: 2023-12-27)

主引用文献

Zhao, L.,Xu, Y.,Chen, M.,Wu, L.,Li, M.,Lu, Y.,Lu, M.,Chen, Y.,Wu, X.
Design of a chimeric glycosyltransferase OleD for the site-specific O-monoglycosylation of 3-hydroxypyridine in nosiheptide.
Microb Biotechnol, 16:1971-1984, 2023

PubMed Abstract: To identify the potential role of the 3-hydroxyl group of the pyridine ring in nosiheptide (NOS) for its antibacterial activity against Gram-positive pathogens, enzymatic glycosylation was utilized to regio-selectively create a monoglycosyl NOS derivative, NOS-G. For this purpose, we selected OleD, a UDP glycosyltransferase from Streptomyces antibioticus that has a low productivity for NOS-G. Activity of the enzyme was increased by swapping domains derived from OleI, both single and in combination. Activity enhancement was best in mutant OleD-10 that contained four OleI domains. This chimer was engineered by site-directed mutagenesis (single and in combination) to increase its activity further, whereby variants were screened using a newly-established colorimetric assay. OleD-10 with I117F and T118G substitutions (FG) had an increased NOS-G productivity of 56%, approximately 70 times higher than that of wild-type OleD. The reason for improved activity of FG towards NOS was structurally attributed to a closer distance (<3 Å) between NOS/sugar donor and the catalytic amino acid H25. The engineered enzyme allowed sufficient activity to demonstrate that the produced NOS-G had enhanced stability and aqueous solubility compared to NOS. Using a murine MRSA infection model, it was established that NOS-G resulted in partial protection within 20 h of administration and delayed the death of infected mice. We conclude that 3-hydroxypyridine is a promising site for structural modification of NOS, which may pave the way for producing nosiheptide derivatives as a potential antibiotic for application in clinical treatment.

PubMed: 37606280
DOI: 10.1111/1751-7915.14332

実験手法

X-RAY DIFFRACTION (2.43 Å)