7XUO

Structure of ATP7B C983S/C985S/D1027A mutant with cisplatin in presence of ATOX1

7XUO の概要

• エントリーDOI: 10.2210/pdb7xuo/pdb
• EMDBエントリー: 33476
• 分子名称: Copper-transporting ATPase 2, PLATINUM (II) ION (2 entities in total)
• 機能のキーワード: p-type cu(+) transporter, atox1, translocase, cisplatin, metal transport, translocate
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 162228.59

構造登録者

Yang, G.,Xu, L.,Chang, S.,Guo, J.,Wu, Z. (登録日: 2022-05-19, 公開日: 2023-04-26, 最終更新日: 2025-07-02)

主引用文献

Yang, G.M.,Xu, L.,Wang, R.M.,Tao, X.,Zheng, Z.W.,Chang, S.,Ma, D.,Zhao, C.,Dong, Y.,Wu, S.,Guo, J.,Wu, Z.Y.
Structures of the human Wilson disease copper transporter ATP7B.
Cell Rep, 42:112417-112417, 2023

PubMed Abstract: The P-type ATPase ATP7B exports cytosolic copper and plays an essential role in the regulation of cellular copper homeostasis. Mutants of ATP7B cause Wilson disease (WD), an autosomal recessive disorder of copper metabolism. Here, we present cryoelectron microscopy (cryo-EM) structures of human ATP7B in the E1 state in the apo, the putative copper-bound, and the putative cisplatin-bound forms. In ATP7B, the N-terminal sixth metal-binding domain (MBD6) binds at the cytosolic copper entry site of the transmembrane domain (TMD), facilitating the delivery of copper from the MBD6 to the TMD. The sulfur-containing residues in the TMD of ATP7B mark the copper transport pathway. By comparing structures of the E1 state human ATP7B and E2-P state frog ATP7B, we propose the ATP-driving copper transport model of ATP7B. These structures not only advance our understanding of the mechanisms of ATP7B-mediated copper export but can also guide the development of therapeutics for the treatment of WD.

PubMed: 37074913
DOI: 10.1016/j.celrep.2023.112417

実験手法

ELECTRON MICROSCOPY (3.6 Å)