7XUG

cryo-EM structure of HK022 putRNA-less E.coli RNA polymerase elongation complex

7XUG の概要

• エントリーDOI: 10.2210/pdb7xug/pdb
• EMDBエントリー: 33468
• 分子名称: non-template DNA, template DNA, RNA (nun gene and immunity region), ... (9 entities in total)
• 機能のキーワード: bacterial transcription, hk022 put, anti-termination, anti-pausing, cryo-em, transcription
• 由来する生物種: Escherichia coli (strain K12); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 532400.55

構造登録者

Hwang, S.,Kang, J.Y. (登録日: 2022-05-18, 公開日: 2022-08-10, 最終更新日: 2024-07-03)

主引用文献

Hwang, S.,Olinares, P.D.B.,Lee, J.,Kim, J.,Chait, B.T.,King, R.A.,Kang, J.Y.
Structural basis of transcriptional regulation by a nascent RNA element, HK022 putRNA.
Nat Commun, 13:4668-4668, 2022

PubMed Abstract: Transcription, in which RNA polymerases (RNAPs) produce RNA from DNA, is the first step of gene expression. As such, it is highly regulated either by trans-elements like protein factors and/or by cis-elements like specific sequences on the DNA. Lambdoid phage HK022 contains a cis-element, put, which suppresses pausing and termination during transcription of the early phage genes. The putRNA transcript solely performs the anti-pausing/termination activities by interacting directly with the E.coli RNAP elongation complex (EC) by an unknown structural mechanism. In this study, we reconstituted putRNA-associated ECs and determined the structures using cryo-electron microscopy. The determined structures of putRNA-associated EC, putRNA-absent EC, and σ-bound EC suggest that the putRNA interaction with the EC counteracts swiveling, a conformational change previously identified to promote pausing and σ might modulate putRNA folding via σ-dependent pausing during elongation.

PubMed: 35970830
DOI: 10.1038/s41467-022-32315-y

実験手法

ELECTRON MICROSCOPY (3.57 Å)