7XTO

Structure of ClA2 reveal the Mechanism of soil bacterial derived chlorinase

7XTO の概要

• エントリーDOI: 10.2210/pdb7xto/pdb
• 分子名称: soil bacterial derived chlorinase, 1-DEAZA-ADENOSINE (3 entities in total)
• 機能のキーワード: chlorinase, sam, clda, fda, antibiotic, oxidoreductase
• 由来する生物種: Umezawaea tangerina
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 175885.24

構造登録者

Liu, Y.H.,Liu, Y.,Li, Y. (登録日: 2022-05-17, 公開日: 2023-01-25, 最終更新日: 2023-11-29)

主引用文献

Liu, Y.,Zhang, H.,Xiao, H.,Li, Y.,Liu, Y.
Expression, purification and structure determination of the chlorinase ClA2.
Biochem.Biophys.Res.Commun., 628:64-67, 2022

PubMed Abstract: Halogenated compounds are particularly important in pharmaceutical and agrochemical products. Biochemical halogenation with halogenases are environmental friendly reactions with high efficiency. Recently, the two new chlorinases (ClA1 and ClA2) were discovered from soil bacteria. However, the protein structure of ClA2 was not identified. Here, we determined the high-resolution crystal structure of ClA2. This structure will help us to understand the catalytic mechanism of chlorinase, and explain the catalytic process of the coupled chlorinase-fluorinase system, which offers the prospect of arising rapid radiolabeling protocols under mild conditions.

PubMed: 36081280
DOI: 10.1016/j.bbrc.2022.08.081

実験手法

X-RAY DIFFRACTION (2.48 Å)