7XTD

RNA polymerase II elongation complex transcribing a nucleosome (EC58oct)

これはPDB形式変換不可エントリーです。

7XTD の概要

• エントリーDOI: 10.2210/pdb7xtd/pdb
• EMDBエントリー: 33447
• 分子名称: DNA-directed RNA polymerase subunit, RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III, RNA polymerase II subunit B12.5, ... (33 entities in total)
• 機能のキーワード: chromatin, nucleosome, transcription, transcription-dna-rna complex, transcription/dna/rna
• 由来する生物種: Komagataella phaffii; 詳細
• タンパク質・核酸の鎖数: 35
• 化学式量合計: 1603524.60

構造登録者

Ehara, H.,Kujirai, T.,Shirouzu, M.,Kurumizaka, H.,Sekine, S. (登録日: 2022-05-16, 公開日: 2022-09-07, 最終更新日: 2024-07-03)

主引用文献

Ehara, H.,Kujirai, T.,Shirouzu, M.,Kurumizaka, H.,Sekine, S.I.
Structural basis of nucleosome disassembly and reassembly by RNAPII elongation complex with FACT.
Science, 377:eabp9466-eabp9466, 2022

PubMed Abstract: During gene transcription, RNA polymerase II (RNAPII) traverses nucleosomes in chromatin, but the mechanism has remained elusive. Using cryo-electron microscopy, we obtained structures of the RNAPII elongation complex (EC) passing through a nucleosome in the presence of the transcription elongation factors Spt6, Spn1, Elf1, Spt4/5, and Paf1C and the histone chaperone FACT (facilitates chromatin transcription). The structures show snapshots of EC progression on DNA mediating downstream nucleosome disassembly, followed by its reassembly upstream of the EC, which is facilitated by FACT. FACT dynamically adapts to successively occurring subnucleosome intermediates, forming an interface with the EC. Spt6, Spt4/5, and Paf1C form a "cradle" at the EC DNA-exit site and support the upstream nucleosome reassembly. These structures explain the mechanism by which the EC traverses nucleosomes while maintaining the chromatin structure and epigenetic information.

PubMed: 35981082
DOI: 10.1126/science.abp9466

実験手法

ELECTRON MICROSCOPY (3.9 Å)