7XRU

Drimenyl diphosphate synthase D303E from Streptomyces showdoensis in complex with 2-fluorofarnesyl diphosphate (2F-FPP) and Mg2+

Summary for 7XRU

• Entry DOI: 10.2210/pdb7xru/pdb
• Related: 7XQ4 7XQZ 7XR7 7XRA
• Descriptor: SQHop_cyclase_C domain-containing protein, (2E,6E)-2-fluoro-3,7,11-trimethyldodeca-2,6,10-trien-1-yl trihydrogen diphosphate, MAGNESIUM ION, ... (4 entities in total)
• Functional Keywords: terpene synthases, type ii tss, biosynthetic protein
• Biological source: Streptomyces showdoensis
• Total number of polymer chains: 1
• Total formula weight: 55551.13

Authors

Pan, X.M.,Du, W.Y.,Yang, Q.,Zhang, B.,Dong, L.B. (deposition date: 2022-05-11, release date: 2023-03-15, Last modification date: 2023-11-29)

Primary citation

Pan, X.,Du, W.,Zhang, X.,Lin, X.,Li, F.R.,Yang, Q.,Wang, H.,Rudolf, J.D.,Zhang, B.,Dong, L.B.
Discovery, Structure, and Mechanism of a Class II Sesquiterpene Cyclase.
J.Am.Chem.Soc., 144:22067-22074, 2022

PubMed Abstract: Terpene cyclases (TCs), extraordinary enzymes that create the structural diversity seen in terpene natural products, are traditionally divided into two classes, class I and class II. Although the structural and mechanistic features of class I TCs are well-known, the corresponding details in class II counterparts have not been fully characterized. Here, we report the genome mining discovery and structural characterization of two class II sesquiterpene cyclases (STCs) from . These drimenyl diphosphate synthases (DMSs) are the first STCs shown to possess β,γ-didomain architecture. High-resolution X-ray crystal structures of DMS from (SsDMS) in complex with both a farnesyl diphosphate and Mg unveiled an induced-fit mechanism, with an unprecedented Mg binding mode, finally solving one of the lingering questions in class II TC enzymology. This study supports continued genome mining for novel bacterial TCs and provides new mechanistic insights into canonical class II TCs that will lead to advances in TC engineering and synthetic biology.

PubMed: 36416740
DOI: 10.1021/jacs.2c09412

Experimental method

X-RAY DIFFRACTION (2.5 Å)