7XRT
Bacteroides thetaiotaomicron ferulic acid esterase (BT_4077)
Summary for 7XRT
| Entry DOI | 10.2210/pdb7xrt/pdb |
| Related | 7XRV |
| Descriptor | Ferulic acid esterase, CALCIUM ION, (4S)-2-METHYL-2,4-PENTANEDIOL, ... (4 entities in total) |
| Functional Keywords | esterase, hydrolase |
| Biological source | Bacteroides thetaiotaomicron VPI-5482 |
| Total number of polymer chains | 10 |
| Total formula weight | 334862.63 |
| Authors | Du, G.M.,Wang, Y.L.,Xin, F.J. (deposition date: 2022-05-11, release date: 2023-11-22, Last modification date: 2024-11-06) |
| Primary citation | Du, G.,Wang, Y.,Zhang, Y.,Yu, H.,Liu, S.,Ma, X.,Cao, H.,Wei, X.,Wen, B.,Li, Z.,Fan, S.,Zhou, H.,Xin, F. Structural insights into the oligomeric effects on catalytic activity of a decameric feruloyl esterase and its application in ferulic acid production. Int.J.Biol.Macromol., 253:126540-126540, 2023 Cited by PubMed Abstract: Oligomeric feruloyl esterase (FAE) has great application prospect in industry due to its potentially high stability and fine-tuned activity. However, the relationship between catalytic capability and oligomeric structure remains undetermined. Here we identified and characterized a novel, cold-adapted FAE (BtFae) derived from Bacteroides thetaiotaomicron. Structural studies unraveled that BtFae adopts a barrel-like decameric architecture unique in esterase families. By disrupting the interface, the monomeric variant exhibited significantly reduced catalytic activity and stability toward methyl ferulate, potentially due to its impact on the flexibility of the catalytic triad. Additionally, our results also showed that the monomerization of BtFae severely decreased the ferulic acid release from de-starched wheat bran and insoluble wheat arabinoxylan by 75 % and 80 %, respectively. Collectively, this study revealed novel connections between oligomerization and FAE catalytic function, which will benefit for further protein engineering of FAEs at the quaternary structure level for improved industrial applications. PubMed: 37634773DOI: 10.1016/j.ijbiomac.2023.126540 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.007 Å) |
Structure validation
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