7XRI

Feruloyl esterase mutant -S106A

7XRI の概要

• エントリーDOI: 10.2210/pdb7xri/pdb
• 関連するPDBエントリー: 7XRH
• 分子名称: Cinnamoyl esterase, ethyl (2E)-3-(4-hydroxy-3-methoxyphenyl)prop-2-enoate (3 entities in total)
• 機能のキーワード: feruloyl esterase mutant (s101a) complexed with ethyl ferulate, hydrolase
• 由来する生物種: Lactobacillus acidophilus
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 115221.45

構造登録者

Hwang, J.S.,Lee, J.H.,Do, H.,Lee, C.W. (登録日: 2022-05-10, 公開日: 2023-05-17, 最終更新日: 2023-11-29)

主引用文献

Jeon, S.,Hwang, J.,Do, H.,Le, L.T.H.L.,Lee, C.W.,Yoo, W.,Lee, M.J.,Shin, S.C.,Kim, K.K.,Kim, H.W.,Lee, J.H.
Feruloyl Esterase ( La Fae) from Lactobacillus acidophilus : Structural Insights and Functional Characterization for Application in Ferulic Acid Production.
Int J Mol Sci, 24:-, 2023

PubMed Abstract: Ferulic acid and related hydroxycinnamic acids, used as antioxidants and preservatives in the food, cosmetic, pharmaceutical and biotechnology industries, are among the most abundant phenolic compounds present in plant biomass. Identification of novel compounds that can produce ferulic acid and hydroxycinnamic acids, that are safe and can be mass-produced, is critical for the sustainability of these industries. In this study, we aimed to obtain and characterize a feruloyl esterase (Fae) from . Our results demonstrated that Fae reacts with ethyl ferulate and can be used to effectively produce ferulic acid from wheat bran, rice bran and corn stalks. In addition, xylanase supplementation was found to enhance Fae enzymatic hydrolysis, thereby augmenting ferulic acid production. To further investigate the active site configuration of Fae, crystal structures of unliganded and ethyl ferulate-bound Fae were determined at 2.3 and 2.19 Å resolutions, respectively. Structural analysis shows that a Phe34 residue, located at the active site entrance, acts as a gatekeeper residue and controls substrate binding. Mutating this Phe34 to Ala produced an approximately 1.6-fold increase in Fae activity against -nitrophenyl butyrate. Our results highlight the considerable application potential of Fae to produce ferulic acid from plant biomass and agricultural by-products.

PubMed: 37446348
DOI: 10.3390/ijms241311170

実験手法

X-RAY DIFFRACTION (2.19 Å)