7XQL

complex structure of LegA15 with GNP

7XQL の概要

• エントリーDOI: 10.2210/pdb7xql/pdb
• 分子名称: Ankyrin repeat-containing protein, PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER (3 entities in total)
• 機能のキーワード: legionella penumophila effector, gtpase, toxin
• 由来する生物種: Legionella pneumophila subsp. pneumophila str. Philadelphia 1
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 104427.56

構造登録者

Chen, T.T.,Lin, Y.L. (登録日: 2022-05-07, 公開日: 2023-05-17, 最終更新日: 2023-12-20)

主引用文献

Chen, T.T.,Lin, Y.,Zhang, S.,Liu, S.,Song, L.,Zhong, W.,Luo, Z.Q.,Han, A.
Atypical Legionella GTPase effector hijacks host vesicular transport factor p115 to regulate host lipid droplet.
Sci Adv, 8:eadd7945-eadd7945, 2022

PubMed Abstract: The intracellular bacterial pathogen uses hundreds of effector proteins to manipulate multiple processes of the host cells to establish a replicative niche known as -containing vacuole (LCV). Biogenesis of the LCV has been known to depend on host small guanosine triphosphatases (GTPases), but whether bacterial effector GTPases are also involved remains unknown. Here, we show that an ankyrin repeat containing effector LegA15 localizes directly in host lipid droplets (LDs), leading to Golgi apparatus fragmentation of the host cells by hijacking the host vesicular transport factor p115. LegA15 is a GTPase with a unique catalytic mechanism, unlike any eukaryotic small GTPases. Moreover, the effector LegA15 co-opts p115 to modulate homeostasis of the host LDs in its GTPase-dependent manner. Together, our data reveal that an atypical GTPase effector regulates the host LDs through impeding the vesicle secretion system of the host cells for intracellular life cycle of .

PubMed: 36525490
DOI: 10.1126/sciadv.add7945

実験手法

X-RAY DIFFRACTION (2.272 Å)