7XPV

crysteal structure of MtdL-S228A-His soaked with GDP-Fucf and Mn

7XPV の概要

• エントリーDOI: 10.2210/pdb7xpv/pdb
• 分子名称: Transglycosylse, [[(2~{R},3~{S},4~{R},5~{R})-5-(2-azanyl-6-oxidanyl-purin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{S},3~{S},4~{S},5~{R})-3,4-bis(oxidanyl)-5-[(1~{S})-1-oxidanylethyl]oxolan-2-yl] hydrogen phosphate, MANGANESE (II) ION, ... (4 entities in total)
• 機能のキーワード: transferase
• 由来する生物種: Marinactinospora thermotolerans
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 89012.62

構造登録者

Li, F.D.,He, C. (登録日: 2022-05-05, 公開日: 2023-03-29, 最終更新日: 2023-11-29)

主引用文献

Du, X.,Chu, X.,Liu, N.,Jia, X.,Peng, H.,Xiao, Y.,Liu, L.,Yu, H.,Li, F.,He, C.
Structures of the NDP-pyranose mutase belonging to glycosyltransferase family 75 reveal residues important for Mn 2+ coordination and substrate binding.
J.Biol.Chem., 299:102903-102903, 2023

PubMed Abstract: Members of glycosyltransferase family 75 (GT75) not only reversibly catalyze the autoglycosylation of a conserved arginine residue with specific NDP-sugars but also exhibit NDP-pyranose mutase activity that reversibly converts specific NDP-pyranose to NDP-furanose. The latter activity provides valuable NDP-furanosyl donors for glycosyltransferases and requires a divalent cation as a cofactor instead of FAD used by UDP-D-galactopyranose mutase. However, details of the mechanism for NDP-pyranose mutase activity are not clear. Here we report the first crystal structures of GT75 family NDP-pyranose mutases. The novel structures of GT75 member MtdL in complex with Mn and GDP, GDP-D-glucopyranose, GDP-L-fucopyranose, GDP-L-fucofuranose, respectively, combined with site-directed mutagenesis studies, reveal key residues involved in Mn coordination, substrate binding, and catalytic reactions. We also provide a possible catalytic mechanism for this unique type of NDP-pyranose mutase. Taken together, our results highlight key elements of an enzyme family important for furanose biosynthesis.

PubMed: 36642179
DOI: 10.1016/j.jbc.2023.102903

実験手法

X-RAY DIFFRACTION (2.4 Å)