7XPO

Crystal Structure of UDP-Glc/GlcNAc 4-Epimerase with NAD/UDP-Glc

7XPO の概要

• エントリーDOI: 10.2210/pdb7xpo/pdb
• 分子名称: UDP-glucose 4-epimerase, NICOTINAMIDE-ADENINE-DINUCLEOTIDE, URIDINE-5'-DIPHOSPHATE-GLUCOSE, ... (5 entities in total)
• 機能のキーワード: epimerase, complex, nad, udp-glc, plant protein
• 由来する生物種: Zea mays (Maize)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 80758.77

構造登録者

Chen, Y.H.,Wang, X.C.,Zhang, C.R. (登録日: 2022-05-05, 公開日: 2023-05-17, 最終更新日: 2023-12-06)

主引用文献

Zhou, Y.,Zhang, T.,Wang, X.,Wu, W.,Xing, J.,Li, Z.,Qiao, X.,Zhang, C.,Wang, X.,Wang, G.,Li, W.,Bai, S.,Li, Z.,Suo, Y.,Wang, J.,Niu, Y.,Zhang, J.,Lan, C.,Hu, Z.,Li, B.,Zhang, X.,Wang, W.,Galbraith, D.W.,Chen, Y.,Guo, S.,Song, C.P.
A maize epimerase modulates cell wall synthesis and glycosylation during stomatal morphogenesis.
Nat Commun, 14:4384-4384, 2023

PubMed Abstract: The unique dumbbell-shape of grass guard cells (GCs) is controlled by their cell walls which enable their rapid responses to the environment. The molecular mechanisms regulating the synthesis and assembly of GC walls are as yet unknown. Here we have identified BZU3, a maize gene encoding UDP-glucose 4-epimerase that regulates the supply of UDP-glucose during GC wall synthesis. The BZU3 mutation leads to significant decreases in cellular UDP-glucose levels. Immunofluorescence intensities reporting levels of cellulose and mixed-linkage glucans are reduced in the GCs, resulting in impaired local wall thickening. BZU3 also catalyzes the epimerization of UDP-N-acetylgalactosamine to UDP-N-acetylglucosamine, and the BZU3 mutation affects N-glycosylation of proteins that may be involved in cell wall synthesis and signaling. Our results suggest that the spatiotemporal modulation of BZU3 plays a dual role in controlling cell wall synthesis and glycosylation via controlling UDP-glucose/N-acetylglucosamine homeostasis during stomatal morphogenesis. These findings provide insights into the mechanisms controlling formation of the unique morphology of grass stomata.

PubMed: 37474494
DOI: 10.1038/s41467-023-40013-6

実験手法

X-RAY DIFFRACTION (1.25 Å)