7XOZ

Crystal structure of RPPT-TIR

7XOZ の概要

• エントリーDOI: 10.2210/pdb7xoz/pdb
• 分子名称: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase, ADENOSINE-5-DIPHOSPHORIBOSE, ... (4 entities in total)
• 機能のキーワード: nlr, plant protein, plant immune signaling, hydrolase
• 由来する生物種: Arabidopsis thaliana (thale cress); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 76886.08

構造登録者

Song, W.,Jia, A.,Huang, S.,Chai, J. (登録日: 2022-05-02, 公開日: 2023-11-08)

主引用文献

Jia, A.,Huang, S.,Song, W.,Wang, J.,Meng, Y.,Sun, Y.,Xu, L.,Laessle, H.,Jirschitzka, J.,Hou, J.,Zhang, T.,Yu, W.,Hessler, G.,Li, E.,Ma, S.,Yu, D.,Gebauer, J.,Baumann, U.,Liu, X.,Han, Z.,Chang, J.,Parker, J.E.,Chai, J.
TIR-catalyzed ADP-ribosylation reactions produce signaling molecules for plant immunity.
Science, 377:eabq8180-eabq8180, 2022

PubMed Abstract: Plant pathogen-activated immune signaling by nucleotide-binding leucine-rich repeat (NLR) receptors with an N-terminal Toll/interleukin-1 receptor (TIR) domain converges on Enhanced Disease Susceptibility 1 (EDS1) and its direct partners, Phytoalexin Deficient 4 (PAD4) or Senescence-Associated Gene 101 (SAG101). TIR-encoded nicotinamide adenine dinucleotide hydrolase (NADase) produces signaling molecules to promote exclusive EDS1-PAD4 and EDS1-SAG101 interactions with helper NLR subclasses. In this work, we show that TIR-containing proteins catalyze adenosine diphosphate (ADP)-ribosylation of adenosine triphosphate (ATP) and ADP ribose (ADPR) through ADPR polymerase-like and NADase activity, forming ADP-ribosylated ATP (ADPr-ATP) and ADPr-ADPR (di-ADPR), respectively. Specific binding of ADPr-ATP or di-ADPR allosterically promotes EDS1-SAG101 interaction with helper NLR N requirement gene 1A (NRG1A) in vitro and in planta. Our data reveal an enzymatic activity of TIRs that enables specific activation of the EDS1-SAG101-NRG1 immunity branch.

PubMed: 35857644
DOI: 10.1126/science.abq8180

実験手法

X-RAY DIFFRACTION (2.52 Å)