7XKM

Crystal structure of DNA-Ag(I) rod comprising a one-dimensional array of 11 silver ions

7XKM の概要

• エントリーDOI: 10.2210/pdb7xkm/pdb
• 分子名称: DNA (5'-D(*CP*GP*CP*GP*CP*(BRU)P*CP*(BRU)P*CP*GP*CP*G)-3'), SILVER ION (3 entities in total)
• 機能のキーワード: dna, silver, rod
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 17461.29

構造登録者

Kondo, J.,Ono, A.,Atsugi, T. (登録日: 2022-04-20, 公開日: 2022-07-06, 最終更新日: 2024-05-29)

主引用文献

Atsugi, T.,Ono, A.,Tasaka, M.,Eguchi, N.,Fujiwara, S.,Kondo, J.
A Novel Ag I -DNA Rod Comprising a One-Dimensional Array of 11 Silver Ions within a Double Helical Structure.
Angew.Chem.Int.Ed.Engl., 61:e202204798-e202204798, 2022

PubMed Abstract: DNA/RNA duplexes containing metal-ion-mediated base pairs (metallo-base pairs) have potential applications in developing nucleic acid-based nanodevices and genetic code expansion. Many metallo-base pairs are formed within duplexes stabilized by Watson-Crick base pairs. Recently, the crystal structure of an Ag -DNA nanowire with an uninterrupted one-dimensional silver array was determined. Here, we present a new DNA helical wire, the "Ag -DNA rod", containing an uninterrupted array of 11 Ag ions. The Ag -DNA rod consisted of only C-Ag -C, G-Ag -G, G-Ag -5-bromouracil ( U), and U-Ag - U metallo base pairs, with no Watson-Crick pairs. The Ag -DNA rods were connected by non-canonical G-G pairs in crystals. Notably, data from our absorbance, circular dichroism, nuclear magnetic resonance, and mass spectrometry analyses suggested that the Ag -DNA rods formed in solution, as well as within crystals.

PubMed: 35641885
DOI: 10.1002/anie.202204798

実験手法

X-RAY DIFFRACTION (2.79 Å)