7XFW

Crystal structure of the ternary complex of Peptidoglycan recognition protein, PGRP-S with hexanoic and tartaric acids at 2.07 A resolution.

「6J3W」から置き換えられました

7XFW の概要

• エントリーDOI: 10.2210/pdb7xfw/pdb
• 分子名称: Peptidoglycan recognition protein 1, L(+)-TARTARIC ACID, 1,2-ETHANEDIOL, ... (8 entities in total)
• 機能のキーワード: cpgrp-s, pgrp-s, immune system
• 由来する生物種: Camelus dromedarius (Arabian camel)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 77289.34

構造登録者

Maurya, A.,Singh, P.K.,Viswanathan, V.,Sharma, P.,Sharma, S.,Singh, T.P. (登録日: 2022-04-02, 公開日: 2022-05-11, 最終更新日: 2023-11-29)

主引用文献

Maurya, A.,Sharma, P.,Singh, P.K.,Viswanathan, V.,Kaur, P.,Sharma, S.,Singh, T.P.
Structure of the complex of camel peptidoglycan recognition protein-S with hexanoic acid reveals novel features of the versatile ligand-binding site at the dimeric interface.
Biochim Biophys Acta Proteins Proteom, 1871:140887-140887, 2022

PubMed Abstract: The short peptidoglycan recognition protein (PGRP-S) of the innate immune system recognizes the invading microbes through binding to their cell wall molecules. In order to understand the mode of binding of PGRP-S to bacterial cell wall molecules, the structure of the complex of camel PGRP-S (CPGRP-S) with hexanoic acid has been determined at 2.07 Å resolution. Previously, we had reported the structures of CPGRP-S in the native unbound state as well as in the complexed forms with the components of various bacterial cell wall molecules such as peptidoglycan (PGN), lipopolysaccharide (LPS), lipoteichoic acid (LTA), mycolic acid (MA) and other fatty acids. These structures revealed that CPGRP-S formed two homodimers which were designated as A-B and CD dimers. It also showed that the fatty acids bind to CPGRP-S in the binding site at the A-B dimer while the non-fatty acids were shown to bind at the interfaces of both A-B and CD dimers. The present structure of the complex of CPGRP-S with hexanoic acid (HA) showed that HA binds to CPGRP-S at the interface of CD dimer. HA was located in the same groove at the CD interface which was occupied by non-fatty acids such as PGN, LPS and LTA and interacts with residues from both C and D molecules. HA is firmly held in the groove with several hydrogen bonds and a number of van der Waals contacts. This is the first structure which reports the binding of a fatty acid in the cleft at the interface of CD dimer.

PubMed: 36596432
DOI: 10.1016/j.bbapap.2022.140887

実験手法

X-RAY DIFFRACTION (2.07 Å)