7XFP

Crystal structure of Helicobacter pylori IceA2

7XFP の概要

• エントリーDOI: 10.2210/pdb7xfp/pdb
• 分子名称: IceA2 protein, SULFATE ION, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: virulence, unknown function
• 由来する生物種: Helicobacter pylori
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 43288.69

構造登録者

Cho, H.Y.,Song, W.S.,Yoon, S.I. (登録日: 2022-04-02, 公開日: 2022-12-21, 最終更新日: 2024-05-29)

主引用文献

Cho, H.Y.,Cho, H.,Song, W.S.,Yoon, S.I.
Structural analysis of the virulence gene protein IceA2 from Helicobacter pylori.
Biochem.Biophys.Res.Commun., 612:162-168, 2022

PubMed Abstract: Helicobacter pylori is a pathogenic bacterium that causes gastric ulcers and cancer. Among the diverse virulence genes of H. pylori, the IceA gene was identified to be expressed upon adherence to host cells. The IceA gene has two alleles, iceA1 and iceA2, which encode completely different proteins. IceA1 protein was shown to exert endonuclease activity, whereas IceA2 has never been analyzed at the molecular level. Based on a sequence analysis, IceA2 proteins differ in length depending on the strain and are classified into five groups (A-E). To structurally characterize IceA2, we determined the crystal structure of group-D IceA2 (IceA2) and performed a modeling-based comparative analysis of IceA2 groups. IceA2 consists of three β-sheet repeats and serially arranges them like the β-propeller structure of the WD40 domain. However, each β-sheet of IceA2 is stabilized using a unique structural motif that is not observed in WD40. Moreover, IceA2 lacks an additionally appended β-strand and does not form the Velcro-like closure of WD40. Therefore, IceA2 adopts a curved rod-like structure rather than an enclosed circular structure in WD40. IceA2 proteins contain 1-4 β-sheet modules depending on the groups and are modeled to be highly diverse in size and shape.

PubMed: 35526497
DOI: 10.1016/j.bbrc.2022.04.090

実験手法

X-RAY DIFFRACTION (1.87 Å)