7XEJ

SufS with D-cysteine for 5 min

7XEJ の概要

• エントリーDOI: 10.2210/pdb7xej/pdb
• 分子名称: Cysteine desulfurase SufS, (2~{S})-2-[(~{E})-[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylideneamino]-3-sulfanyl-propanoic acid, 1,2-ETHANEDIOL, ... (6 entities in total)
• 機能のキーワード: cysteine desulfurase, inhibitor, biosynthetic protein
• 由来する生物種: Bacillus subtilis subsp. subtilis str. 168
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 47891.95

構造登録者

Nakamiura, R.,Fujishiro, T. (登録日: 2022-03-31, 公開日: 2023-04-05, 最終更新日: 2025-04-02)

主引用文献

Nakamura, R.,Fujishiro, T.
Visualizing thiazolidine ring formation in the reaction of D-cysteine and pyridoxal-5'-phosphate within L-cysteine desulfurase SufS.
Biochem.Biophys.Res.Commun., 754:151497-151497, 2025

PubMed Abstract: The reactivity of pyridoxal-5'-phosphate (PLP) with cysteine and its derivatives has been of increasing interest because the corresponding product, a thiazolidine PLP-cysteine adduct, can be formed via PLP-dependent enzymatic and non-enzymatic reactions. Here, we report biochemical and X-ray crystallographic snapshots of thiazolidine formation in reaction of D-cysteine with PLP in SufS, a PLP-dependent L-cysteine desulfurase. By comparing L- and D-penicillamine-bound SufS showing no thiazolidine formation in the crystals with D-cysteine SufS, we proposed a thiazolidine formation mechanism with important factors: the polar environments provided by the carbonyl groups of Ala28-Ala29 and Lys224-mediated base catalysis for the nucleophilic thiolate of D-cysteine.

PubMed: 40020322
DOI: 10.1016/j.bbrc.2025.151497

実験手法

X-RAY DIFFRACTION (1.74 Å)