7X8A

Cryo-EM structure of a bacterial protein complex

7X8A の概要

• エントリーDOI: 10.2210/pdb7x8a/pdb
• 関連するPDBエントリー: 7X7A 7X7R
• EMDBエントリー: 33040 33046 33056
• 分子名称: CHAT domain protein, RAMP superfamily protein, RNA (33-MER), ... (4 entities in total)
• 機能のキーワード: complex, rna-binding, rna binding protein, rna binding protein-rna complex, rna binding protein/rna
• 由来する生物種: Candidatus Scalindua brodae; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 291039.60

構造登録者

Yu, G.,Wang, X.,Deng, Z.,Zhang, H. (登録日: 2022-03-11, 公開日: 2022-11-16, 最終更新日: 2025-06-25)

主引用文献

Yu, G.,Wang, X.,Zhang, Y.,An, Q.,Wen, Y.,Li, X.,Yin, H.,Deng, Z.,Zhang, H.
Structure and function of a bacterial type III-E CRISPR-Cas7-11 complex.
Nat Microbiol, 7:2078-2088, 2022

PubMed Abstract: The type III-E CRISPR-Cas system uses a single multidomain effector called Cas7-11 (also named gRAMP) to cleave RNA and associate with a caspase-like protease Csx29, showing promising potential for RNA-targeting applications. The structural and molecular mechanisms of the type III-E CRISPR-Cas system remain poorly understood. Here we report four cryo-electron microscopy structures of Cas7-11 at different functional states. Cas7-11 has four Cas7-like domains, which assemble into a helical filament to accommodate CRISPR RNA (crRNA), and a Cas11-like domain facilitating crRNA-target RNA duplex formation. The Cas7.1 domain is critical for crRNA maturation, whereas Cas7.2 and Cas7.3 are responsible for target RNA cleavage. Target RNA binding induces the structural arrangements of Csx29, potentially exposing the catalytic site of Csx29. These results delineate the molecular mechanisms underlying pre-crRNA processing, target RNA recognition and cleavage for Cas7-11, and provide a structural framework to understand the role of Csx29 in type III-E CRISPR system.

PubMed: 36302881
DOI: 10.1038/s41564-022-01256-z

実験手法

ELECTRON MICROSCOPY (2.8 Å)