7X7H

Crystal structure of Fructose regulator/Histidine phosphocarrier protein complex from Vibrio cholerae

7X7H の概要

• エントリーDOI: 10.2210/pdb7x7h/pdb
• 分子名称: Catabolite repressor/activator, HPr family phosphocarrier protein, CALCIUM ION, ... (4 entities in total)
• 機能のキーワード: pts, vibrio, fructose, transcriptional regulator, galr/laci, hpr, gene regulation, gene regulation-transferase complex, gene regulation/transferase
• 由来する生物種: Vibrio cholerae; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 92048.62

構造登録者

Kim, M.-K.,Zhang, J.,Yoon, C.-K.,Seok, Y.-J. (登録日: 2022-03-09, 公開日: 2023-03-15, 最終更新日: 2024-05-29)

主引用文献

Yoon, C.K.,Lee, S.H.,Zhang, J.,Lee, H.Y.,Kim, M.K.,Seok, Y.J.
HPr prevents FruR-mediated facilitation of RNA polymerase binding to the fru promoter in Vibrio cholerae.
Nucleic Acids Res., 51:5432-5448, 2023

PubMed Abstract: Phosphorylation state-dependent interactions of the phosphoenolpyruvate (PEP):carbohydrate phosphotransferase system (PTS) components with transcription factors play a key role in carbon catabolite repression (CCR) by glucose in bacteria. Glucose inhibits the PTS-dependent transport of fructose and is preferred over fructose in Vibrio cholerae, but the mechanism is unknown. We have recently shown that, contrary to Escherichia coli, the fructose-dependent transcriptional regulator FruR acts as an activator of the fru operon in V. cholerae and binding of the FruR-fructose 1-phosphate (F1P) complex to an operator facilitates RNA polymerase (RNAP) binding to the fru promoter. Here we show that, in the presence of glucose, dephosphorylated HPr, a general PTS component, binds to FruR. Whereas HPr does not affect DNA-binding affinity of FruR, regardless of the presence of F1P, it prevents the FruR-F1P complex from facilitating the binding of RNAP to the fru promoter. Structural and biochemical analyses of the FruR-HPr complex identify key residues responsible for the V. cholerae-specific FruR-HPr interaction not observed in E. coli. Finally, we reveal how the dephosphorylated HPr interacts with FruR in V. cholerae, whereas the phosphorylated HPr binds to CcpA, which is a global regulator of CCR in Bacillus subtilis and shows structural similarity to FruR.

PubMed: 36987873
DOI: 10.1093/nar/gkad220

実験手法

X-RAY DIFFRACTION (2 Å)