7X55

A Cbc-ParM filament with GTP and a short incubation time

7X55 の概要

• エントリーDOI: 10.2210/pdb7x55/pdb
• EMDBエントリー: 33008
• 分子名称: ParM/StbA family protein, GUANOSINE-5'-TRIPHOSPHATE (2 entities in total)
• 機能のキーワード: parm actin-like plasmid segregation, cell cycle
• 由来する生物種: Clostridium botulinum
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 165342.66

構造登録者

Koh, A.,Ali, S.,Robinson, R.,Narita, A. (登録日: 2022-03-04, 公開日: 2023-03-08, 最終更新日: 2025-07-16)

主引用文献

Ali, S.,Koh, A.,Popp, D.,Tanaka, K.,Kitaoku, Y.,Miyazaki, N.,Iwasaki, K.,Mitsuoka, K.,Robinson, R.C.,Narita, A.
Bacterial genome-encoded ParMs.
J.Biol.Chem., 301:110351-110351, 2025

PubMed Abstract: ParMs generally exist on low-copy number plasmids where they contribute to plasmid segregation and stable inheritance. We carried out bioinformatics analysis, which indicated that ParM genes are not only confined to plasmids but are also occasionally found on genomes. Here we report the discovery and characterization of two chromosome-encoded ParMs (cParMs) from the genomes of Desulfitobacterium hafniense (Dh-cParM1) and Clostridium botulinum (Cb-cParM). Both cParMs form filaments, exhibit nucleotide hydrolysis, and possess characteristic ParM subunit structures. Dh-cParM1 forms single and tightly coupled double filaments and is highly conserved on the chromosomes of five of six Desulfitobacterium species. Interestingly, these bacteria have not been reported to harbor plasmids. Cb-cParM possesses unique properties. Its filaments were stable after nucleotide hydrolysis and Pi release, and its ParR (Cb-cParR) did not affect the initial phase of Cb-cParM polymerization but displayed properties of a depolymerization factor for mature filaments. These results indicate functional, polymerizing ParMs can be encoded on genomes, suggesting that ParM roles may extend to other functions beyond plasmid segregation.

PubMed: 40499760
DOI: 10.1016/j.jbc.2025.110351

実験手法

ELECTRON MICROSCOPY (8.6 Å)